The use of RAPD marker for verification of Dendrobium hybrid, D. santana x D. friedericksianum orchid

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Published: May 15, 2019
Keywords:
Orchid Dendrobium hybrid RAPD primer

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Choopeng, S.
Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand.
Te-chato, S.
Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand.
Khawnium, T.
Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand.

Abstract

Dendrobium is one of the most important orchids due to its beautiful flower. The use of random amplified polymorphic DNA (RAPD) marker for verification of Dendrobium hybrid orchid between D. santana and D. friedericksianum was investigated. Twelve 10-mer primers of RAPD were used for the above purpose. The results showed that 6 primers which were OPA03, OPB07, OPB18, OPJ04, OPR11 and OPT06 could amplify and give a clear profiles of DNA from all samples. The primer OPA03240, 310, OPB07380, 420,850, OPJ04550, 980, OPT06310, 450, 620, OPR11180, 500, 1,040, OPB18300, 410, 560,1,010 could be used as markers for verification of hybrid between D. santana and D. friedericksianum.

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How to Cite
Choopeng, S., Te-chato, S., & Khawnium, T. (2019). The use of RAPD marker for verification of Dendrobium hybrid, D. santana x D. friedericksianum orchid. International Journal of Agricultural Technology, 15(3), 399–408. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/8227
Issue
Vol. 15 No. 3 (2019): May
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

Antony, J. J. J., Poobathy, R., Danial, M., Sundarasekar, J. and Subramaniam, S. (2012). Polymorphism analysis of cryopreserved Dendrobium Bobby Messina protocorm-like bodies (PLBs) using RAPD markers. Plant Omics, 5:427-431.

Benner, M. S., Braunstein, M. D. and Weisberg, M. U. (1995). Detection of DNA polymorphisms within the genus Cattleya (Orchidaceae). Plant Molecular Biology Reporter, 13:147-155.

Chattopadhyay, P., Banerjee, N. and Chaudhary, B. (2012). Genetic characterization of selected medicinal Dendrobium (Orchidaceae) species using molecular markers. Research Journal of Biology, 2:117-125.

Feng, S., Zhao, H., Lu, J., Liu, J., Shen, B. O. and Wang, H. (2013). Preliminary genetic linkage maps of Chinese herb Dendrobium nobile and D. moniliforme. Journal of Genetics, 92:205-212.

Ferreira, W. D. M., Kerbauy, G. B. and Pimentel Costa, A. P. (2006). Micropropagation and genetic stability of a Dendrobium hybrid (Orchidaceae). In Vitro Cellular and Developmental Biology-Plant, 42:568-571.

Goh, M. W. K., Kumar, P. P., Lim, S. H. and Tan, H. T. W. (2005). Random amplified polymorphic DNA analysis of the moth orchids, Phalaenopsis (Epidendroideae: Orchidaceae). Euphytica, 141:11-22.

Inthawong, S., Bundithya, W., Kuanprasert, N. and Apavatjrut, P. (2006). Analysis of Intersectional Hybrids of Dendrobium by RAPD Technique. Kasetsart Journal (Natural Science), 40:456-461.

Jheng, C. F., Chen, T. C., Lin, J. Y., Chen, T. C., Wu, W. L. and Chang, C. C. (2012). The comparative chloroplast genomic analysis of photosynthetic orchids and developing DNA markers to distinguish Phalaenopsis orchids. Plant science, 190:62-73.

Khosravi, A. R., Kadir, M. A., Kadzemin, S. B., Zaman, F. Q. and De Silva, A. E. (2009). RAPD analysis of colchicine induced variation of the Dendrobium Serdang beauty. African Journal of Biotechnology, 8:1455-1465.

Kishor, R. and Sharma, G. J. (2009). Intergeneric hybrid of two rare and endangered orchids, Renanthera imschootiana Rolfe and Vanda coerulea Griff. ex L. (Orchidaceae): Synthesis and characterization. Euphytica, 165:247-256.

Kishor, R., Devi, H. S., Jeyaram, K. and Singh, M. R. K. (2008). Molecular characterization of reciprocal crosses of Aerides vandarum and Vanda stangeana (Orchidaceae) at the protocorm stage. Plant Biotechnology Report, 2:145-152.

Leitch, I. J., Kahandawala, I., Suda, J., Hanson, L., Ingrouille, M. J., Chase, M. W. and Fay, M. F. (2009). Genome size diversity in orchids: consequences and evolution. Annals of botany, 104:469-481.

Lim, S. H., Teng, P. C. P, Lee, Y. H. and GOH, C. J. (1999). RAPD analysis of some species in the genus Vanda (Orchidaceae). Annals of Botany, 83:193-196.

Niknejad, A., Kadir, M. A., Kadzimin, S. B., Abdullah, N. A. P. and Sorkheh, K. (2009). Molecular characterization and phylogenetic relationships among and within species of Phalaenopsis (Epidendroideae: Orchidaceae) based on RAPD analysis. African Journal of Biotechnology, 8:5225-5240.

Parab, G. V., Krishnan, S., Janarthanam, M. K., Sivaprakash, K. R., and Parida, A. (2008). ISSR and RAPD markers assessed genetic variation of Aerides maculosum—An epiphytic orchid from Goa, India. Journal of plant biochemistry and biotechnology, 17:107-109.

Pinheiro, L. R., Rabbani, A. R. C., da Silva, A. V. C., da Silva Lédo, A., Pereira, K. L. G. and Diniz, L.E.C. (2012). Genetic diversity and population structure in the Brazilian Cattleya labiata (Orchidaceae) using RAPD and ISSR markers. Plant Systematics and Evolution, 298:1815-1825.

Poobathy, R., Xavier, R., Sinniah, U. R. and Subramaniam, S. (2013). Molecular stability of protocorm-like bodies of Dendrobium Sonia-28 after encapsulation-dehydration and vitrification. Australian Journal of Crop Science, 7:189-195.

Xue, D., Feng, S., Zhao, H., Jiang, H., Shen, B., Shi, N. and Wang, H. (2010). The linkage maps of Dendrobium species based on RAPD and SRAP markers. Journal of Genetics and Genomics, 37:197-204.