Study on vaginal epithelial cells in Brahman cattle suspected reach puberty
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Abstract
Puberty can be characterized by the time when the cattle first estrus. Estrus in cattle can be observed through the cytology of the vaginal epithelial (Vaginal smear). The vaginal wall cytology of Brahman cattle predicted at the onset of puberty was studied. Brahman cattles were developed by Livestock Breeding Center for Excellence and Forage (BPTU-HPT) Sembawa since 2012. The estimation of puberty was using 3 nonlinear mathematical models, there are consisted of Gompertz (10.28 months, n = 4), Bertalanffy (09.96 months, n = 4), and Logistics (15.5 months, n = 7). Vaginal smear was done by smearing wet cotton bud using aquadest on Brahman cattle vaginal wall, then it was smeared to glass object. The object glasses were immersed in alcohol (70%) for 5 to 7 minutes, then it were soaked in liquid Giemsa stain (5%) for 45 minutes. The object glasses were dried, then it were observed using a microscope with a magnification 40 times. The vaginal smear samples were taken 8 times and done every 3 days for each cattles. The results described that there were only parabasal and intermediates cell in all ages of cattle observed. All the animal observation was not entered the age of puberty.
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