Comparison of toxic effects of Psidium guajava leaf and bark extracts against Brine Shrimp (Artemia salina)

Main Article Content

Bautista, M. L.
Pomer, P.
Salonga, J. J.
Tolentino, J. J.

Abstract

Psidium guajava, also known as guava, is a medicinal plant that mainly found in tropical or subtropical areas like the Philippines. This plant has been used in treating different diseases and is also known for its medicinal and antimicrobial properties. Despite this, there are only a few numbers of studies regarding the toxicology of P. guajava. In this study, Brine Shrimp Lethality Assay (BSLA) was done to identify if the leaf and bark extracts of P. guajava have toxic effects against Artemia salina (brine shrimp). Results showed bark extracts having an LC50 value of 480.14 µg/mL and leaf extracts with an LC50 value of 949.13 µg/mL, and the mortality rates of brine shrimps for the bark extracts were relatively higher than the leaf extracts. It revealed that P. guajava bark extracts were more toxic than the leaf extracts.

Article Details

How to Cite
Bautista, M. L., Pomer, P., Salonga, J. J., & Tolentino, J. J. (2018). Comparison of toxic effects of Psidium guajava leaf and bark extracts against Brine Shrimp (Artemia salina). International Journal of Agricultural Technology, 14(7), 1033–1038. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/8470
Section
Original Study

References

Barbalho, S. M., Farinazzi-Machado, F. V., Goulart, R. A., Brunnati, A. S., Ottoboni, A. B. and Nicolau, C. T. (2012). Psidium guajava (guava): a plant of multipurpose medicinal applications. Medicinal and Aromatic Plants. 1:2167-0412.

Biswas, B., Rogers, K., Mclaughlin, F., Daniels, D. and Yadav, A. (2013). Antimicrobial activities of leaf extracts of guava (Psidium guajava L.) on two gram-negative and gram-positive bacteria. International Journal of Microbiology. pp. 1-7.

Clarkson, C., Maharaj, V. J., Crouch, N. R., Grace, O. M., Pillay, P., Matsabisa, M. G., Bhagwandin, N., Smith, P. J. and Folb, P. I., (2004). In vitro antiplasmodial activity of medicinal plants native to or naturalized in South Africa. Journal of Ethnopharmacology. 92:177-191.

Guintu, Ferdinand (2013). Effectivity of guava leaves (Psidium guajava) as mouthwash for patients with aphthous ulcers. Philippine Journal of Otolaryngology Head and Neck Surgery. 2:8-13.

Gutiérrez, R. M., Mitchell, S. and Solis, R. V. (2008). Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology. 117:1-27.

Luber, J., Palmieri, M. J., Botelho, C. M., Rinaldo, D. and Andrade-Vieira, L. F. (2015). Investigation on the effects of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa. Anais Da Academia Brasileira De Ciências. 87:903-913.

Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E. and McLaughlin, J. L. (1982). Brine Shrimp: A convenient general bioassay for active plant constituents. Planta Medica. 45:31-34.

Skibola, C. F. and Smith, M. T. (2000). Potential health impacts of excessive flavonoid intake. Free Radical Biology and Medicine. 29:375-383.

Sukandar, E. Y., Kurniati, N. F. and Fitria, V. (2014). Evaluation of teratogenicity effects of ethanolic extracts of binahong leaves (Anredera Cordifolia (Ten) steenis) in wistar rat. International Journal of Pharmacy and Pharmaceutical Sciences. 6:422-426.

Wu, C. (2014). An important player in brine shrimp lethality bioassay: The solvent. Journal of Advanced Pharmaceutical Technology and Research. 5:57-58.