Effects of BA and NAA on plant regeneration of neck orange (Citrus reticulate Blanco)
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Abstract
Neck orange (Citrus reticulate Blanco) is native orange that very popular in southern of Thailand. It had health benefits and also used in many industries of citrus processing. There are expansion of the plantation area to increase the production of sufficient to demand of consumers. The branches are currently not enough to cultivation. This study showed the effect of benzyladenine (BA) and naphthalene acetic acid (NAA) to develop a new plant of Neck orange. The seeds of orange were surface sterilized with sodium hypochlorite and culturing on Murashige and Skoog (MS) medium supplemented with BA (0-2 mg/L) and NAA (0-1 mg/L), 3% sucrose, with and without 0.2% activated charcoal for 6 weeks. The result revealed that MS free medium (without plant growth regulators) gave the highest percentage of germination (83.93 percent). The highest of Shoot length (3.17 cm) were obtain from MS medium containing 0.5 mg/L BA adding activated charcoal, whereas MS medium with 2.0 mg/L BA gave the highest number of shoot (3.33 shoots/seed) and MS medium with 0.5 mg/L NAA, 0.5 mg/L BA adding activated charcoal gave the highest root length (21.30 cm).
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