Induction of shoot and root from nodes of Kadsura heteroclita

Abstract

Results described on shoot and root induction from node of medicinal plant Kadsura heteroclita. The sterilized nodes were used as explants and cultured on solid synthetic medium, Murashige and Skoog (MS) medium supplemented with single or combined Plant Growth Regulators (PGRs) of 6-benzylaminopurine (BAP), meta-Topolin (mT), Indole-3-butyric acid (IBA),1-Naphthaleneacetic acid (NAA) and Gibberellin (GA₃) were applied. The best result for shoot induction was shown on the medium supplemented with 0.5 mg/L of mT after 8 weeks. Transferred shoot to MS medium supplemented with combination PGRs of 0.5 mg/L of mT and 0.5, 1.0, 1.5 and 2.0 mg/L of IBA NAA or GA₃ after 16 weeks revealed that the explant were developed to multiple shoots which averaged number of shoot were 3 shoots/explant.  The roots were not perfectly exhibited like sore on medium supplemented with 0.5 mg/L of mT and 1.5 mg/L of GA₃. The high concentration of Auxin inhibited growth roots.  The root induction was done by cutting the shoot and cultured on MS medium supplemented with combination PGRs of 0.5 mg/L of mT and 0.01, 0.02, 0.03, 0.04 and 0.05 mg/L of IBA after 4 weeks found that root length was approximately 2 mm on 0.5 mg/L of mT and 0.05 mg/L of IBA.