Effect of plant growth regulators on micropropagation of Vanilla aphylla and Vanilla planifolia sp. Variegate
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Abstract
The plant growth regulators for propagating large number of vanillas in a short period of time was reported. Axillary buds were used from the explants of Vanilla aphylla and Vanilla planifolia sp. variegata that cultured on solid Gamborg’s B5 medium supplemented with 0, 0.5, 1, 1.5 and 2 mg/L 6-benzylaminopurine (BA) for 8 weeks. The result showed that the highest average number of shoot proliferation and root induction were obtained at 1 mg/L BA of 3.2 cm. and 5.3 cm. respectively for Vanilla aphylla. Vanilla planifolia sp. variegata showed the highest shoot elongation of 1.9 cm. at 0.5 mg/L BA. Root induction was the highest of 0.9 cm. at 2 mg/L BA. After 12 weeks of culture The explants were grew the best after 12 weeks. They were transplanted into pots and covered by plastic bags for acclimatization and has maintained in greenhouse.
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References
Abebe, Z., Mengesha, A., Teressa, A. and Tefera, W. (2009). Efficient in vitro multiplication protocol for Vanilla planifolia using nodal explants in Ethiopia. African Journal of Biotechnology. 8:6817-6821.
Asikin, D., Motohashi, T. and Kondo, K. (2014). Micropropagation and chromosome stability of four endangered species of orchids in Indonesia. International society of chromosome botany. 9:89-96.
Ayele, Y., Tefera, W. and Bantte, K. (2017). Enhanced protocol development for in vitro multiplication and rooting of vanilla (Vanilla planifolia Andrews.) clone (Van.2/05). Biotechnology Journal International. 18:1-11.
Baskaran, P. and Jayabalan, N. (2005) In vitro plant regeneration and mass propagation system for Sorghum bicolor a valuable major cereal crop. Journal of Agricultural Technology. 12:345-363.
Bhattacharjee, B. and Islam, S. (2014) Effects of plant growth regulators on multiple shoot induction in Vanda tessellata (Roxb.) Hook. Ex G.Don an endangered medical orchid. International Journal of Science and Nature. 5:707-712.
Biradar, V. Inamdar, A. Shamse, A and Patil, M. (2016). In vitro studies on the influence of different concentrations of growth regulators on economically important orchid, Vanilla planifolia. International Journal of Current Microbiology and Applied Sciences. 5:311-323.
Chalermglin, P. (2015). How to make a vanilla. Kehakaset Magazine. 39:180-185.
Fhasaiwanmai. (2018). Vanilla is wonder orchids. Retrieved 25 August 2018. https://www.bloggang.com/.
Halim, R., Akyol, B. and Gurel, A. (2017). In vitro regeneration of vanilla. Journal of Applied Biological Sciences. 11:5-10.
Haque, S. and Ghosh, B. (2017). Regeneration of cytologically stable plants through dedifferentiation, redifferentiation, and artificial seeds in Spathoglottis plicata blume. (Orchidaceae). Horticulture Plant Journal 3:199-208.
Khunsri, S. (2009). In vitro Propagation and Genetic Diversity Among Vanilla Species. (Master Thesis). King Mongkut’s Institute of Technology Ladkrabang, Thailand.
Sruamsiri, P. (1986). Vanilla: Spice crops. Division of Horticulture, Faculty of Agriculture, Chiang Mai University, pp. 209.
Tan, B., Chin, C. and Alderson, P. (2011). Optimisation of plantlet regeneration from leaf and nodal derived callus of Vanilla planifolia Andrews. Plant Cell Tissue and Organ Culture. 105:457-463.
Zhang, A., Wang, H., Shao, Q., Xu, M., Zhang, W. and Li, M. (2013). Large scale in vitro propagation of Anoectochilus roxburghii for commercial application: Pharmaceutically important and ornamental plant. Industrial Crops and Products. 70:158-162.
Zuraida, A., Izzati, K., Nazreena, O., Zaliha, W., Radziah, C., Zamri, Z. and Sreeramanan, S. (2013). A simple and efficient protocol for the mass propagation of Vanilla planifolia. Amarican Journal of Plant Sciences. 4:1685-1692.
