Schiff base synthesis, formulation as emulsifiable concentrate and study its nematicidal efficiency on root-knot nematode Meloidogyne spp. under greenhouse conditions
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Abstract
The reaction of primary amines with carbonyl compounds has been recognized as a source of Schiff bases. The biological activity of new imines (Schiff bases) against Meloidogyne incognita was investigated. According to the bioassay, all investigated chemicals had promised as nematicidal effectiveness. Standard approach was used to synthesize Schiff base from salicylic aldehyde (2-hydroxybenzaldehyde) and anthranilic acid (2-aminobenzoic acid). Spectral analysis revealed its chemical structure. The Schiff base physio-chemical characteristics were investigated. The results revealed that emulsifiable concentrate (EC) was the best formulation form. It was developed as 10% emulsifiable concentrate. The new formula's physio-chemical properties were investigated. It was tested against root-knot nematode, Meloidogyne spp. in the second-stage larvae under greenhouse conditions. The new formula significantly decreased the amount of galls/root, eggs, and limited the larvae's ability to penetrate eggplant roots, with a direct proportion between concentration and effect in both cases. The novel formulation showed 38.4, 92.8, 99.3, and 100 percent gall reduction and 52.6, 94.7, 100, and 100 percent egg reduction at 10, 100, 1000, and 10000 ppm, respectively. The new EC formulation might be used to control the root-knot nematode, Meloidogyne spp.
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References
Abd-Alla, H. I. and Hamouda, S. E. S. (2021). Study of potential activity of clove oil 10 % emulsifiable concentrate formulation on Two-spotted spider Mite Tetranychus urticae Koch (Acari: Tetranychidae). Journal of Applied and Natural Science, 13:1414-1419.
Abolusoro, S. A., Abolusoro, P. F., Mathew, F. O. and Izuogu, N. B. (2013). Effects of Organic and Inorganic Manures on the Growth Attributes of Root-knot Nematode (Meloidogyne Incognita) Infected Ethopian Egg Plant (Solanum Aethiopicum). World Journal of Agricultural Research, 1:104-107.
Adaji, M. U., Wuana, R. A., Itodo, A. U., Eneji, I. S. and Iorungwa, M. S. (2021). Nematicidal Activity of Cu (II) and Fe (II) Schiff Base Ligand Complexes: Synthesis and Characterization. FUAM Journal of Pure and Applied Sciences, 1:99-109.
American Society of Testing Materials (ASTM). (2001). Standard test method for surface & interfacial tension of solution D-1331.
American Society of Testing Materials ASTM). (2005). Standard test method for rheological properties of non - Newtonian materials by rotational (Brookfield type) Viscometer, D-2196 Copyright ASTM, Bar Harbor Drive, West Conshohocken, PA 19248-2959, United States.
Berenbaum, M. (2000). Committee on the Future Role of Pesticides, National Academy of Sciences, The Future Role of Pesticides in U.S. Agriculture, National Academy Press, Washington, D. C., p.48.
Castro, C. E. and Thomason, I. J. (1971). Mode of Action of Nematicides. In: Plant Parasitic Nematodes. B. M. Z. Uckerman, W. F. Maiand R. A. Rhode. Acad. Press, Lond. & N. Y., pp.289-296.
Chitwood, D. J. (2003). Research on Plant-Parasitic Nematode Biology Conducted by the United States Department of Agriculture-Agricultural Research Service. Pest Management Science, 59:748-753.
Cimerman, Z., Miljanic, S. and Galic, N. (2000). Schiff Bases Derived from Aminopyridines as Spectrofluorimetric Analytical Reagents. Croatica Chemica Acta, 73:81- 95.
Dobrat, W. and Martijn, A. (1995). Collaborative International Pesticides Analytical Council CIPAC hand book Vol. F, Physicochemical Methods for Technical and Formulated Pesticides.
El-Deeb, A. M., El-Sappah, A. H. and Arisha, M., H., (2018). Efficiency of Some Bionematicides against Root-Knot Nematode Meloidogyne incognita on Three Tomato Cultivars under Greenhouse Conditions. Zagazig Journal of Agricultural Research, 45:2001-2010.
El-Sisi, A., El-Mageed, A., El-Asawi, T. F. and El-Sharkawy, R. (2011). Improvement the Physico-chemical Properties and Efficiency of Some Insecticides Formulation by Using Adjuvants Against Cotton Leafworm Spodoptera littoralis (BOISD.). Journal of Plant Protection and Pathology, 2:757-764.
Escobar, C., Barcala, M., Cabrera, J. and Fenoll, C. (2015). Overview of root-knot nematodes and giant cells. In Advances in botanical research, 73, 1-32. Academic Press. doi: https://doi.org/10.1016/bs.abr.2015.01.001
FAO/WHO (2010). Manual on Development and Use of FAO and WHO Specifications for Pesticides, 1st Ed. 3rd Rev. FAO Plant Production and Protection, FAO, Rome, MT 36.3
Fishel, F. M. (2010). Pesticide formulations. EDIS, (4).
Glazer, I., Salame, L. and Segal, D. (1997). Genetic enhancement of nematicide resistance inentomopathogenic nematodes. Biocontrol Science and Technology, 7:499-512.
Hala, M. I., Hanan, S. T. and Naglaa, K. Y. (2016). Effect of Storage on the Stability and Biological Effectiveness of Some Insecicides. Journal of Biological Chemistry and Environmental Sciences, 11:265-282.
Ibrahim, H. S., Hamouda, S. E. S., El-kady, A. M. A. and Abd-Alla, H. I. (2014). Study the Nematicidal Efficiency of Corchorus Olitorius, Cinnamomum Camphora, Portulace Oleraceae and Lantana Camara Extracted Saponins and Their Formulations on Root-Knot Nematodes Meloidogyne spp. Nature and Science, 12:40-45.
Kampfe, L. and Schütze, H. (1995). Reactions of Rhabditis Oxycerca After Long-Term Exposure To Aldicarb and Oxamyl. Iii: Altered Bionomic and Physiological Reactions. Nematologica, 41:449-467.
Kundua, A., Shakil, N. A., Saxena, D. B., Kumar, J. and Walia, S. (2009). Microwave Assisted Solvent-Free Synthesis and Biological Activities of Novel Imines (Schiff bases). Journal of Environmental Science and Health, 44:428-34.
Libs, E. S. and Salim, E. H., (2017). Formulation of Essential Oil Pesticides Technology and their Application. Agricultural Research & Technology, 9:42-60.
Lynch, M. and Griffin, W. (1974). Food Emulsions in: Emulsion Technology, by Lissant KJ, Marcell Decker, Inc. New York. Mukerjee P. and KJ Mysels (1971) Critical Micelle Concentration of Aqueous Surfactant Systems. National Bureau of Standards Washington DC, 1-21.
Mitkowski, N. A. and Abawi, G. S. (2003). Root-Knot Nematodes. Plant Health Instructor, Online publication. doi: 10.1094/PHII-2003-0917-01.
Moens, M., Perry, R. N. and Starr, J. L. (2009). Meloidogyne species a diverse group of novel and important plant parasites. Root-Knot Nematodes, 1:483. doi:10.1079/9781845934927.0001
Nelson, F. C. and Fiero, G. W. (1954). Pesticide Formulations, A Selected Aromatic Fraction Naturally Occurring in Petroleum as a Pesticide Solvent. Journal of Agricultural and Food Chemistry, 2:735-737.
Nicol, J. M., Turner, S. J., Coyne, D., Nijs, L. D., Hockland, S. and Maafi, Z. T. (2011) Current nematode threats to World Agriculture. In Genomics and Molecular Genetics of Plant-Nematode Interactions; Springer Science and Business Media LLC: Dordrecht, The Netherlands, pp.21-43.
Osipow L. I. (1964). Surface Chemistry Theory and Application. Reinhold Publishing Crop, New York, 4736-39.
Pereira, V. J., da Cunha, J. P. A. R., de Morais, T. P., de Oliveira, J. P. R. and de Morais, J. B. (2016). Physical-chemical properties of pesticides: concepts, applications, and interactions with the environment. Bioscience Journal, 32:627-641.
Pesticide Formulation (2017). Retried from http://s3.amazonaws.com/nasda2/media/Reports/Core-Chpt-4.
Raman, N., Pothiraj, K. and Baskaran, T. (2011). DNA Interaction, Antimicrobial, Electrochemical and Spectroscopic Studies of Metal (II) Complexes with Tridentate Heterocyclic Schiff Base Derived from 2-Methyl Acetoacetanilide, Journal of Molecular Structure, 1000:135-144.
Soliman, N. M. T. (2005). Evaluation the Pesticidal Action of Some Formulation of Plant Extracts; 111 PP. MS.C. Thesis, Institute of Environmental Studies and Research, Ain Shams University.
Spanoghe, P., De Schampheleire, M., Van der Meeren, P. and Steurbaut, W. (2007). Influence of agricultural adjuvants on droplet spectra. Pest Management Science: formerly Pesticide Science, 63:4-16.
Taylor, A. L. and Sasser, J. N. (1978). Biology, Identification and Control of Root-Knot Nematodes (Meloidogyne spp.) 111 p. Raleigh, North Carolina: Coop. Pub. Dept. Plant Pathol. North Carolina State University and US Agency Int. Dev.
World Health Organization, WHO. (1979). Specification of Pesticides Used in Public Health, 5th Ed. Geneva.
Xavier, A. and Srividhya N. (2014). Synthesis and Study of Schiff Base Ligands, Journal of Applied Chemistry, 7:06-15.