Effects of microbial fermented liquid (MFL) supplementation on gas production kinetics and digestibility using in vitro gas production technique
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Abstract
The effects of microbial fermented liquid (MFL) supplementation on rumen fermentation and digestibility of dairy steer using in vitro gas production technique was studied. The two rumen fistulated dairy steers as a rumen fluid donor was used. The levels of source of microbes (yeast (Y) and microbial fermented liquid (MFL) were incorporated with the levels of supplementation (0, 10, 20 and 30 % of concentrate) was recorded. It was found that the intercept value (a) and IVDMD and IVOMD were interacted (p<0.01) between microbial source and supplement levels, while, supplement levels affected on a and the insoluble fraction (b), microbial source affected on b, potential extent of gas production (a+b) and cumulative gas production at 72 h and IVOMD. Moreover, supplementation of MFL with 20 % of concentrate were the highest (p<0.05) of b, c, a+b, cumulative gas production at 72 h, IVDMD and IVOMD. In conclusion, supplementation of MFL could improve nutritional digestibility and a possible productivity in ruminants.
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References
AOAC (1995). Official methods of analysis, 16th ed. Association of Official Analytical Chemists; Arlington. VA, USA.
Carvalho, B. F., Aacute, C. L. S., Vila, Pinto, J. C. and Schwan, R. F. (2013). Effect of propionic acid and Lactobacillus plantarum UFLA SIL 1 on the sugarcane silage with and without calcium oxide. African Journal of Microbiology Research. 7:4159-4168.
Contreras-Govea, F. E., Muckb, R. E., Mertensb, D. R. and Weimerb, P. J. (2004). Microbial inoculant effects on silage and in vitro ruminal fermentation and microbial biomass estimation for alfalfa, BMR corn, and corn silages. Animal Feed Science and Technology. 163:2-10.
Dolezal, P., Dolezal, J., Szwedziak, K., Dvoracek, J., Zeman, L., Tukiendorf, M. and Havlicek, Z. (2012), Use of Yeast Culture in the TMR of Dairy Holstein Cows. Iranian Journal of Applied Animal Science. 2:51-56.
Elghandour, M. M. Y., Salem, A. Z. M., Castañeda, J. S. M., Camacho, A. E. K. and Chagoyán, J. C. V. (2015). Direct-fed microbes: A tool for improving the utilization of low quality roughages in ruminants. Journal of Integrative Agriculture. 14:526-533.
Ellis, J. L., Bannink, A., Hindrichsen, I. K., Kinley, R. D., Pellikaan, W. F., Milora, N.and Dijkstra, J. (2016). The effect of lactic acid bacteria included as probiotic or silage inoculant on in vitro rumen digestibility, total gas and methane production. Animal Feed Science and Technology. 211:61-74.
Ghazanfar, S., Anjum, M. I., Azim, A. and Ahmed, I. (2015). Effects of dietary supplementation of yeast (Saccharomyces cerevisiae) culture on growth performance, blood parameters, Nutrient digestibility and fecal flora of dairy heifers. The Journal of Animal & Plant Sciences. 25:53-59.
Khonyoung, D. and Wonnakom, P. (2010). Utilization of vertiver Grass for ruminant diet. Animal Science. Maejo University. Thailand.
Kung, Jr., Stokes, L., Lin, M. R. and C. J. (2003). Silage additives. In: Buxton, D. R., Muck, R. E., Harrison, J. H. (Eds.), Silage Science and Technology. Agron. Monogr. 42. ASA, CSSA, and SSSA, Madison, WI, USA. pp. 305-360.
Leicester, H. C. W., Robinson, P. H. and Erasmus, L. J. (2016). Effects of two yeast based direct fed microbials on performance of high producing dairy cows. Animal Feed Science and Technology. 215:58-72.
Lila, Z. A., Mohammed, N., Yasui, T., Kurokawa, Y., Kanda, S. and Itabashi, H. (2004). Effects of a twin strain of Saccharomyces cerevisiae live cells on mixed ruminal microorganism fermentation in vitro1. Journal of Animal Science. 82:1847-1854.
Menke H. H. and Steingass H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development. 28:7-55.
Polyorach, S., Wanapat, M. and Wanapat, S. (2013). Enrichment of protein content in cassava (Manihot esculenta Crantz) by supplementing with yeast for use as animal feed. Emirates Journal of Food and Agriculture. 25:142-149.
Quinn, M. J., May, M. L., Hales, K. E., DiLorenzo, N., Leibovich, J., Smith, D. R. and Galyean, M. L. (2009). Effects of ionophores and antibiotics on in vitro hydrogen sulfide production, dry matter disappearance, and total gas production in cultures with a steam-flaked corn-based substrate with or without added sulfur. Journal of Animal Science. 87:1705-1713.
Ørskov, E. R. and McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science. 92:499-503.
Ramaswami, N., Chaudhary, L. C., Agarwal, N. and Kamra, D. N. (2005). Effect of lactic acid producing bacteria on the performance of male crossbred calves fedroughage based diet. Asian-Australasian Journal of Animal Sciences. 18:1110.
Ridwan, R., Bungsu, W. A., Astuti, W. D., Rohmatussolihat, Sari, N. F., Fidriyanto, R., Jayanegara, A., Wijayanti, I. and Widyastuti, Y. (2018). The use of lactic acid bacteria as ruminant probiotic candidates based on in vitro rumen fermentation characteristics. Buletin Peternakan. 42:31-36.
Rossi, F., Luccia, A. D., Vincenti, D. and Cocconcelli, P. S. (2004). Effects of peptidic fractions from Saccharomyces cerevisiae culture on growth and metabolism of the ruminal bacteria Megasphaera elsdenii. Animal Research. 53:177-186.
SAS Institute Inc (1985). Users Guide: Basics. SAS Institute; Cary, North Carolina, USA Seo, K., Kim, S.W., Kim, M. H., Upadhaya, S. D., Kam, D. K. and Jong K. H. (2010). Direct-fed Microbials for Ruminant Animals. Asian-Australasian Journal of Animal Sciences. 12:1657-1667.
Seo, J. K., Kim, S. W. Kim, M. H. Upadhaya, S. D. Kam, D. K. and Ha, J. K. (2010). Direct-fed microbials for ruminant animals. Asian-Australasian Journal of Animal Sciences. 23:1657-1667.
Steel, R. G. D. and Torrie, J. H. (1980). Principles and Procedures of Statistics. A biometrical approach. 2nd edition. McGraw-Hill, New York, USA. pp. 20-90.
Tang, S. X., Tayo, G. O., Tan, Z. L., Sun, Z. H., Shen, L. X., Zhou, C. S., Xiao, W. J., Ren, G. P., Han, X. F. and Shen, S. B. (2008). Effects of yeast culture and fibrolytic enzyme supplementation on in vitro fermentation characteristics of low-quality cereal straws. Journal of Animal Science. 86:1164-1172.
Tilley, J. M. A. and Terry, R. A. (1963). A two-stage technique for the digestion of forage crops. Journal of the British Grassland Society. 18:104-111.
Weinberg, Z. G., Chen, Y. and Gamburg, M. (2004). The passage of lactic acid bacteria from silage into rumen fluid, in vitro studies. Journal of Dairy Science. 87:3386-3397.
Weinberg, Z., Shatz, O., Chen, Y., osef, E. Y., Nikbahat, M., Ben-Ghedalia, D. and Miron, J. (2007). Effect of lactic acid bacteria inoculants on in vitro digestibility of wheat and corn silages. Journal of Dairy Science. 90:4754-4762.
Worku, B., Workicha, H. and Seneait, G. (2016). Effect of Effective Microbes (EM) bokashi supplementation on weight gain performance of yearling bucks of woito guji goat breeds fed natural hay as basal diet. Advances in Life Science and Technology. 41:36-40.
