In vitro effect of plant growth regulators (PGRs) for callus induction and plant regeneration from suspension of Hamata (Stylosanthes hamata cv. Verano)
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Abstract
The plant regeneration from seed of Hamata (Stylosanthes hamata cv. Verano) was found. Seeds were induced on MS medium (Murashige and Skoog, 1962) with different concentration of cytokinin were used 0.5, 1 and 3 mg/L 6-benzyladenine (BAP) meta-Topolin (mT) or Thidizuron (TDZ), respectively. The result showed that the highest percentage of seed induction was 65% and average shoots 8.66 shoots per seed on MS medium with 3 mg/L mT for 12 weeks cultivation. For node of hamata were study on callus and shoot induction on MS medium with 0.5 mg/L IBA combined with 0.5, 1 and 3 mg/L TDZ. The highest percentage of callus and shoot induction was 100% gave average areas of callus at 11192.84 mm3 and the number of shoots per callus was 13.50 shoots on MS medium with 0.5 mg/L IBA combined 3 mg/L TDZ for 8 weeks cultivation Cell suspension culture of callus that developed from node of hamata. The friable callus was transferred to liquid MS medium with 0.5 mg/L IBA combined with 3 mg/L TDZ for 4 weeks cultivation After that transferred to MS medium with 3 mg/L mT for shoots regeneration. The percentage of shoots generation from callus was shown on 90% and the number of shoots per callus was 14.00 shoots for 12 weeks cultivation. Transferred shoot to root medium containing of MS medium with Indole-3-butyric acid (IBA) Naphthaleneacetic acid (NAA) or Indole-3-acetic acid (IAA) at 0.1, 0.3 and 0.5 mg/L. The result showed that the highest percentage of root induction was 100% and gave averaged roots at 11.00+1.87 roots per shoot on MS medium with 0.3 mg/L NAA for 12 weeks cultivation.
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