Biological control of anthracnose disease on banana var ‘Namwa Mali-Ong’ by Neosartorya species
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Abstract
The anthracnose disease caused by Colletotrichum musae on banana was controlled by biological control agents. Seven isolates of C. musae were collected from banana growing area in Suphanburi, Nakhonpathom, Ratchaburi and Nonthaburi province, Thailand. Pathogenicity was tested on fresh banana, and proved that isolates CMDC-01 and CMNM-01 were most aggressive isolates. These isolates were molecular phylogeny confirmed species. In vitro test was done by dual culture tecnique, two isolates of pathogen were tested against ten isolates of antagonistic fungi (Neosartorya hiratsukae, N. pseudofischeri, N, aureola, N. spinosa, N. fenneliae, Talaromyces muroii and T. trachyspermus). The result showed the best antagonistic isolate was N. pseudofischeri EU13 which inhibited C. musae CMDC-01 of 51.23% and N. fenneliae CHA03-A11 inhibited C. musae CMNM-01 of 59.85%.
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