Antibacterial and anti-tyrosinase activities of the methanolic extracts from leaves of Tectona grandis
Main Article Content
Abstract
The antibacterial and anti-tyrosinase activities of methanolic extracts from fresh and fallen leaves of Tectona grandis (Teak) was studied. Fresh and fallen of teak leaves were collected from Thongphaphum and Phitsanulok silviculture research station. Antibacterial activity was determined by disc diffusion method with concentration at 500 µg/disc. Fresh and fallen of teak leaves extracts showed a good activity against Staphylococcus aureus and Staphylococcus epidermidis. In the other hand, fresh teak leaves inhibited growth of Propionibacterium acnes better than fallen teak leaves. The anti-tyrosinase activity was determined by Dopachrome method with concentration at 1500 µg/ml, inhibitory power of tyrosinase enzyme from fresh and fallen leaves extracts value were detected in the percentage between 35.45%-73.65%.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Aboaba, S., Akande, A. and Flamini, G. (2013). Chemical constituents, toxicity and antimicrobial activities of the essential oil from the leaves of Tectona grandis. Elixir Bio Technology. 61:16795-16798.
Alam, N., Yoon, K. N. Lee, J. S., Cho, H. J. and Lee, T. S. (2012). Consequence of the antioxidant activities and tyrosinase inhibitory effects of various extracts from the fruiting bodies of Pleurotus ferulae. Saudi Journal of Biological Sciences. 19:111-118.
Brahmi, F., Mechri, B., Dhibi, M. and Hammami, M. (2013). Variations in phenolic compounds and antiradical scavenging activity of Olea europaea leaves and fruits extracts collected in two different seasons. Industrial Crops and Products. 49:256-264.
Chang, T. and Tseng, M. (2006). Preliminary screening of soil actinomycetes for anti-tyrosinase activity. Journal of Marine Science and Technology. 14:190-193.
Cushnie, T. and Lamb, A. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents. 26:343-356.
Farjana, A., Zerin, N. and Kabir, M. S. (2014). Antimicrobial activity of medicinal plant leaf extracts against pathogenic bacteria. Asian Pacific Journal of Tropical Disease. 4:920-923.
Ghasemzadeh, A., Jaafar, H. Z. E. and Rahmat, A. (2016). Changes in antioxidant and antibacterial activities as well as phytochemical constituents associated with ginger storage and polyphenol oxidase activity. BMC Complementary and Alternative Medicine. 16:1-11.
Haliloglu, Y., Ozek, T., Tekin, M., Goger, F., Baser, K. H. C. and Ozek, G. (2017). Phytochemicals, antioxidant, and antityrosinase activities of Achillea sivasica Çelik and Akpulat. International Journal of Food Properties. 20:693-706.
Krishna, M. S. and Nair, A. J. (2010). Antibacterial, Cytotoxic and Antioxidant Potential of different extracts from leaf, bark and wood of Tectona grandis. International Journal of Pharmaceutical Sciences and Drug Research. 2:155-158.
Krishna, M. S. and Nair, A. J. (2011). Anthraquinones from leaves of Tectona grandis: A detailed study on its antibacterial activity and other biological properties. International Journal of Phytomedicine. 3:50-58.
Kusnadi, J., Arumingtyas, E. L., Ningtyas, D. W. and Setiawan, E. C. (2016). Antioxidant activity of MAE extracted teak (Tectona grandis L.F.) leaves collected from different plantation site at Java Island, Indonesia International Journal of ChemTech Research. 7:154-160.
Nayeem, N. and Karvekar, M. D. (2011). Isolation of phenolic compounds from the methanolic extract of Tectona grandis. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2:221-225.
Nidavani, R. and Am, M. (2014). Teak (Tectona grandis Linn.): A renowned timber plant with potential medicinal values. International Journal of Pharmacy and Pharmaceutical Sciences. 6:86-90.
Purushotham, K. G., Arun, P., Johnsy Jayarani, J., Vasnthakumari, R., Sankar, L. and Raviprakash Reddy, B. (2010). Synergistic in vitro antibacterial activity of Tectona grandis leaves with tetracycline. International Journal of Pharmtech Research. 2:519-523.
Stacey, A. N. D, Mello, Graeme, J., Finlay, Bruce, C., Baguley, Marjan, E. and Askarian-Amiri. (2016). Signaling Pathways in Melanogenesis. International Journal of Molecular Sciences. 17:1-18.
Sun, L., Guo, Y., Zhang, Y. and Zhuang, Y. (2017). Antioxidant and anti-tyrosinase activities of phenolic extracts from rape bee pollen and inhibitory melanogenesis by cAMP/MITF/TYR pathway in B16 mouse melanoma cells. Frontiers in Pharmacology. 8:1-9.
Tamokoua, J. D. D., Michel, F. T., Hippolyte, K. W., Jules, R. K. and Pierre, T. (2009). Antimicrobial activities of methanol extract and compounds from stem bark of Vismia rubescens. Journal of Ethnopharmacology. 124:571-575.
Tendencia, E. A. (2004). Disk diffusion method. In Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment. Aquaculture department southeast asian fisheries development Center, Tigbauan, Iloilo, Philippine, pp. 13-29.
Thi, N. D. and Hwang, E. S. (2014). Bioactive Compound contents and antioxidant activity in Aronia (Aronia melanocarpa) leaves collected at different growth stages. Preventive Nutrition and Food Science. 19:204-212.
Wikaningtyas, P. and Sukandar, E. Y. (2016). The antibacterial activity of selected plants towards resistant bacteria isolated from clinical specimens. Asian Pacific Journal of Tropical Biomedicine. 6:16-19.