In vitro antimicrobial properties of different solvent extracts from carissa fruts Carissa carandas L.
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Abstract
In vitro antimicrobial activities of various solvent systems (0%, 25%, 50%, 75% and 100% ethanol in water) of carissa fruit extracts (Carissa carandas) was reported. Agar well diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and killing time were determined. The results found that absolute ethanol and 75% ethanol extracts inhibited all 11 strains of both pathogenic and spoilage bacteria at the concentration of 100 mg/ml. The absolute ethanol extract was selected as an optimal extraction solvent for the following study showed the strongest antimicrobial activity. The MIC values of absolute ethanol extract to inhibit Pseudomonas fluorescens TISTR 358, Staphylococcus aureus TISTR 118, Salmonella Typhimurium TISTR 292, Escherichia coli TISTR 780 were 25, 25, 50, and 50 mg/ml, whereas the MBC values to inhibit these bacteria were 100, 25, 50, and 100 mg/ml, respectively. Moreover, S. aureus TISTR 118 and S. Typhimurium TISTR 292 were completely killed at 20 and 6 minutes exposure time by absolute ethanol extract from C. carandas fruits at the concentration of 25 mg/ml and 50 mg/ml, respectively.
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