Isolation and characterization of keratinolytic bacteria from soil samples of poultry waste dumping sites
Article Sidebar
Main Article Content
Abstract
The keratinolytic bacteria isolated from soil samples which containing the degrading feathers was investigated. Thirteen bacterial isolates were selected and were subjected to preliminary screening through protease assay using Milk Agar Medium. Six isolates were detected positive for protease activity and were further characterized via biochemical and microscopic assays. Isolates were grown in basal medium containing feathers as sole nutrient source and the degree of feather degradation was monitored. Two isolates exhibited conspicuous keratinase activity. DNA from these two candidate organisms were isolated and subjected to PCR using 16S rRNA specific primers. PCR products were sequenced and analysis revealed that both of them belong to Bacillus cereus species. Isolation of potential keratinolytic microorganisms could have potential biotechnological used especially in processes which involved keratin hyrdolysis.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Bradbury, J. H. (1973). The structure and chemistry of keratin fibers. Advances in Protein Chemistry. 27:111-211.
Coia, J. and Cubie, H. (1995). Bacillus cereus the immunoassay kit directory. Springer Netherlands. 1:648-649.
Elmayergi, H. H. and Smith, R. E. (1971). Influence of growth of Streptomyces fradiae on pepsin-HCl digestibility and methionine content of feather meal. Canadian Journal of Microbiology. 17:1067-1072.
Jeevana Lakshmi, P., Kumari Chitturi, Ch. M. and Lakshmi, V. V. (2013). Efficient degradation of feather by keratinase producing Bacillus sp. International Journal of Microbiology.Article ID 608321. doi:10.1155/2013/608321.
Kaul, S. and Sumbali, G. (1997). Keratinolysis by poultry farm soil fungi. Mycopathologia. 139:137-40.
Kulkarni, S. A. and Jadhav, A. R. (2014). Isolation and characterization of keratinolytic bacteria from poultry farm soils. International Research Journal of Biological Sciences. 3:29-33.
Lee, C. G., Ferket, P. R. and Shih, J. C. H. (1991). Improvement of feather digestibility by bacterial keratinase as a feed additive. FASEB Journal. 59:1312.
Lin, X., Kelemen, D. W., Miller, E. S. and Shih, J. C. H. (1995). Nucleotide sequence and expression of kerA, the gene encoding akeratinolytic protease of Bacillus licheniformisi. Applied and Environmental Microbiology. 61:1469-1474.
Odetallah, N. H., Wang, J. J., Garlich, J. D. and Shih, J. C. H. (2003). Keratinase in starter diets improve growth of broiler chicks. Poultry Science. 82:664-670.
Parry, D. A. T. and North, A. C. T. (1998). Hard a-keratin intermediate filament chains: substructure of the N- and C-terminal domains and the predicted structure and function of the C-terminal domains of type i and type II chains. Journal of Structural Biology. 122:67-75.
Riffel, A. and Brandelli, A. (2006). Keratinolytic bacteria isolated from feather waste. Brazilian Journal of Microbiology. 37:395-399.
Schaeffer, A. B. and Fulton, M. D. (1933). A simplified method of staining endospores. Science. 17:194.
Williams, C. M., Lee, C. G., Garlich, J. D. and Shih, J. C. H. (1991). Evaluation of a bacterial feather fermentation product, feather-lysate, as a feed protein. Poultry Science. 70:85-94.
Xu, B., Zhong, Q., Tang, X., Yang, Y. and Huang, Z. (2009). Isolation and characterization of a new keratinolytic baterium that exhibits significant feather-degrading capability. African Journal of Biotechnology. 8:4590-4596.
