Effect of emulsifiable concentrate of Metarhizium rileyi (Farl.) Kepler, S.A. Rehner & Humber to third larval instar of fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
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Abstract
Metarhizium rileyi (Farl.) Kepler, S.A. Rehner & Humber is a cosmopolitan entomopathogenic fungus effective against major insect pests of agricultural crops. In the Philippines, this fungus was successfully isolated from mycosed armyworm larvae collected in corn and onion fields. Our previous bioassays showed the virulence of crude fungal suspensions of M. rileyi isolate from onion armyworm (Spodoptera exigua Hübner) to S. exigua and its cross infection to fall armyworm (S. frugiperda J.E. Smith) and true armyworm (Mythimna separata (Walker)). This research work aimed to formulate this entomopathogenic fungus for increased efficacy and stability. Emulsifiable concentrate (EC) of M. rileyi was prepared using various liquid carriers. Exposure to EC formulations resulted to mycosis of third larval instar of S. frugiperda. Storage time and conditions affected the bioefficacy of the EC formulations. Pupation and emergence to adults were also affected. The efficacy and stability of these EC formulations are continuously being evaluated under storage conditions.
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