Characterization of biosynthesized gold nanoparticles from Streptomyces misionensis PYA9 with biomedical and environmental applications
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Abstract
Result showed marine actinobacterium PYA9 isolated from the coastal sediments of Pondicherry, India effectively reduced aqueous chloroauric acid to gold nanoparticles (AuNPs). Characterization of actinobacteria mediated AuNPs was performed using UV-Vis, Particle size analyzer, TEM, FTIR, and XRD. A strong absorbance peak was observed at 539 nm in the visible region confirming the presence of AuNPs. The average size of AuNPs was found to be 61 nm. Images from TEM analysis revealed the presence of spherical and triangular nanoparticles. The biosynthesized AuNPs were screened for their antibacterial activity against Salmonella typhi, Enterococcus faecalis, Staphylococcus aureus and Escherichia coli. Relative to the actinobacterial compounds, the synthesized AuNPs displayed highest activity. The biosynthesized AuNPs also displayed anti-biofouling property when tested against two biofouling bacteria. Taxonomy of the actinobacterial strain PYA9 was identified using 16S rRNA sequencing as Streptomyces misionensis. Our study showed new opportunities of utilizing actinobacterial extracts as a reducing agent for synthesizing biocompatible, large scale AuNPs with potential applications in the fields of medicine and environment
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References
Ahmad, F., Ashraf, N., Ashraf, T., Zhou, R. B. and Yin, D. C. (2019). Biological synthesis of metallic nanoparticles (MNPs) by plants and microbes: their cellular uptake, biocompatibility, and biomedical applications. Applied microbiology and biotechnology, 103:2913-2935.
Aswani, T., Reshmi, S. and Suchithra, T. V. (2019). Actinomycetes: Its Realm in Nanotechnology. In Microbial Nanobionics. Springer, Cham, pp.127-140.
Balagurunathan, R., Radhakrishnan, M., Rajendran, R. B. and Velmurugan, D. (2011). Biosynthesis of gold nanoparticles by actinomycete Streptomyces viridogens strain HM10. Indian Journal of Biochemistry and Biophysics, 48:331-5.
Bhosale, R. S., Hajare, K. Y., Mulay, B., Mujumdar, S. and Kothawade, M. (2015). Biosynthesis, characterization and study of antimicrobial effect of silver nanoparticles by Actinomycetes spp. International Journal of Current Microbiology and Applied Sciences, 2:144-151.
Boroumand, S., Safari, M., Shaabani, E., Shirzad, M. and Faridi-Majidi, R. (2019). Selenium nanoparticles: synthesis, characterization and study of their cytotoxicity, antioxidant and antibacterial activity. Materials Research Express, 6:0850d8.
Edison, L. K. and Pradeep, N. S. (2020). Actinobacterial nanoparticles: green synthesis, evaluation and applications. In Green Nanoparticles. Springer, Cham, pp.371-384.
El-Batal, A. I., Mona, S. S. and Al-Tamie, M. S. S. (2015). Biosynthesis of gold nanoparticles using marine Streptomyces cyaneus and their antimicrobial, antioxidant and antitumor (in vitro) activities. Journal of Chemical and Pharmaceutical Research, 7:1020-1036.
Firdhouse, M. J. and Lalitha, P. (2022). Biogenic Green Synthesis of Gold Nanoparticles and Their Applications–A Review of Promising Properties. Inorganic Chemistry Communications, 109800.
Ghosh, D., Sarkar, D., Girigoswami, A. and Chattopadhyay, N. (2011). A fully standardized method of synthesis of gold nanoparticles of desired dimension in the range 15 nm–60 nm. Journal of nanoscience and nanotechnology, 11:1141-1146.
He, X. and Yang, H. (2013). Au nanoparticles assembled on palygorskite: Enhanced catalytic property and Au–Au2O3 coexistence. Journal of Molecular Catalysis A: Chemical, 379:219-224.
Hutchison, J. E. (2008). Greener nanoscience: a proactive approach to advancing applications and reducing implications of nanotechnology. ACS nano, 2:395-402.
Kabiri, F., Aghaei, S. S., Pourbabaee, A. A., Soleimani, M. and Komeili Movahhed, T. (2022). Antibiofilm and cytotoxic potential of extracellular biosynthesized gold nanoparticles using actinobacteria Amycolatopsis sp. KMN. Preparative Biochemistry and Biotechnology, 1-14.
Khadivi Derakhshan, F., Dehnad, A. and Salouti, M. (2012). Extracellular biosynthesis of gold nanoparticles by metal resistance bacteria: Streptomyces griseus. Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, 42:868-871.
Kumari, S., Tehri, N., Gahlaut, A. and Hooda, V. (2020). Actinomycetes mediated synthesis, characterization, and applications of metallic nanoparticles. Inorganic and Nano-Metal Chemistry, 51:1386-1395.
Li, X., Xu, H., Chen, Z. S. and Chen, G. (2011). Biosynthesis of nanoparticles by microorganisms and their applications. Journal of Nanomaterials, 2011.
Manimaran, M. and Kannabiran, K. (2017). Actinomycetes‐mediated biogenic synthesis of metal and metal oxide nanoparticles: progress and challenges. Letters in applied microbiology, 64:401-408.
Manivasagan, P., Venkatesan, J., Sivakumar, K. and Kim, S. K. (2016). Actinobacteria mediated synthesis of nanoparticles and their biological properties: A review. Critical reviews in microbiology, 42:209-221.
Menon, S., Rajeshkumar, S. and Kumar, V. (2017). A review on biogenic synthesis of gold nanoparticles, characterization, and its applications. Resource-Efficient Technologies, 3:516-527.
Merza, K. S., Al-Attabi, H. D., Abbas, Z. M. and Yusr, H. A. (2012). Comparative study on methods for preparation of gold nanoparticles. Green and Sustainable Chemistry, 2:26-28.
Mohanapriya, M. K., Deshmukh, K., Ahamed, M. B., Chidambaram, K. and Pasha, S. K. (2016). Influence of cerium oxide (CeO2) nanoparticles on the structural, morphological, mechanical and dielectric properties of PVA/PPy blend nanocomposites. Materials Today: Proceedings, 3:1864-1873.
Nagajyothi, P. C., An, T. M., Sreekanth, T. V. M., Lee, J. I., Lee, D. J. and Lee, K. D. (2013). Green route biosynthesis: Characterization and catalytic activity of ZnO nanoparticles. Materials Letters, 108:160-163.
Patil, M. P., Kang, M. J., Niyonizigiye, I., Singh, A., Kim, J. O., Seo, Y. B. and Kim, G. D. (2019). Extracellular synthesis of gold nanoparticles using the marine bacterium Paracoccus haeundaensis BC74171T and evaluation of their antioxidant activity and antiproliferative effect on normal and cancer cell lines. Colloids and Surfaces B: Biointerfaces, 183:110455.
Ramakrishna, M., Rajesh Babu, D., Gengan, R. M., Chandra, S. and Nageswara Rao, G. (2016). Green synthesis of gold nanoparticles using marine algae and evaluation of their catalytic activity. Journal of Nanostructure in Chemistry, 6:1-13.
Ranjitha, V. R. and Rai, V. R. (2017). Actinomycetes mediated synthesis of gold nanoparticles from the culture supernatant of Streptomyces griseoruber with special reference to catalytic activity. 3 Biotech, 7:1-7.
Ravindra, P. (2009). Protein-mediated synthesis of gold nanoparticles. Materials Science and Engineering: B, 163:93-98.
Salem, S. S. and Fouda, A. (2021). Green synthesis of metallic nanoparticles and their prospective biotechnological applications: an overview. Biological Trace Element Research, 199:344-370.
Sayed, R. and Saad, H. (2021). Gold Nanoparticles: Green Synthesis, Characterization and Biological Activities. Egyptian Journal of Chemistry, 64:2-3.
Shanmugasundaram, T., Radhakrishnan, M., Gopikrishnan, V., Pazhanimurugan, R. and Balagurunathan, R. (2013). A study of the bactericidal, anti-biofouling, cytotoxic and antioxidant properties of actinobacterially synthesised silver nanoparticles. Colloids and Surfaces B: Biointerfaces, 111:680-687.
Sharma, D., Kanchi, S. and Bisetty, K. (2019). Biogenic synthesis of nanoparticles: a review. Arabian Journal of Chemistry, 12:3576-3600.
Shivaji, S. W., Arvind, M. D. and Zygmunt, S. (2014). Biosynthesis, optimization, purification and characterization of gold nanoparticles. African journal of microbiology research, 8:138-146.
Składanowski, M., Wypij, M., Laskowski, D., Golińska, P., Dahm, H. and Rai, M. (2017). Silver and gold nanoparticles synthesized from Streptomyces sp. isolated from acid forest soil with special reference to its antibacterial activity against pathogens. Journal of Cluster Science, 28:59-79.
Zonooz, N. F., Salouti, M., Shapouri, R. and Nasseryan, J. (2012). Biosynthesis of gold nanoparticles by Streptomyces sp. ERI-3 supernatant and process optimization for enhanced production. Journal of Cluster Science, 23:375-382.
