Archaeal communities discovered in the phytotelmata of Nepenthes alata Blco. samples obtained from Mt. Makiling, Philippines as revealed by high-throughput molecular sequencing analysis

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Published: Mar 15, 2023
Keywords:
Archaea Mt. Makiling Forest Reserve Nepenthes alata Blco. Pitcher plant Phytotelmata

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Alcantara, N. R.
Institute of Weed Science, Entomology and Plant Pathology, College of Agriculture and Food Science, Los Baños, Laguna, Philippines
Mendoza, B. C.
Microbiology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, Laguna, Philippines
Sabino, N. G.
2Microbiology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, Laguna, Philippines; Museum of Natural History, University of the Philippines Los Baños, Laguna, Philippines
Simbahan, J. F.
Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines
Balatibat, J. B.
Museum of Natural History, University of the Philippines Los Baños, Laguna, Philippines ;Department of Forest Biological Sciences, College of Forestry and Natural Resources, University of the Philippines Los Baños, Laguna, Philippines
de los Reyes, F. L. III
Department of Civil, Construction, and Environmental Engineering, North Carolina State University, Raleigh, North Carolina, USA
Hyman, M. R.
Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA.

Abstract

The archaeome in the phytotelmata of the Philippine endemic pitcher plant, Nepenthes alata Blco. was investigated. The alpha diversity analyses revealed that the ten most abundant archaeal sequences detected were less than 97% similar to known sequences, and were classified under “Others”, indicating the presence of previously uncharacterized archaea. At the genus level, Methanocorpusculum, Candidatus_Nitrosopumilus and Methanimicrococcus were found to be the most abundant in the phytotelm of young pitchers. In contrast, there were no genera of previously identified archaea that were considered abundant in the phytotelm of mature pitchers. Further analyses of archaeal composition over time showed that of the 560 total phylotypes detected in young pitchers, 269 (~ 48.0%) were consistently present across three monthly sampling periods. On the other hand, of the 570 total phylotypes detected in the mature pitchers, 200 (~ 35.1%) were consistently present across the same sampling periods. This paper establishes that N. alata hosts multiple species of archaea regardless of age of the pitchers and implies a high possibility that N. alata-archaeome interactions exist which have not been examined previously.

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How to Cite
Alcantara, N. R., Mendoza, B. C., Sabino, N. G., Simbahan, J. F., Balatibat, J. B., de los Reyes, F. L. III, & Hyman, M. R. (2023). Archaeal communities discovered in the phytotelmata of Nepenthes alata Blco. samples obtained from Mt. Makiling, Philippines as revealed by high-throughput molecular sequencing analysis. International Journal of Agricultural Technology, 19(2), 355–370. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/9466
Issue
Vol. 19 No. 2 (2023): March
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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