Effect of Tomato Seed Priming with Bacillus subtilis on Seed Germination, and Seedling Survival in Outbreak Bacterial Wilt in Greenhouse Condition
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Abstract
Tomato cultivation is often suffered from bacterial wilt (Ralstonia solanacearum), an important soil-born plant disease. B. subtilis is an antagonist bacterium of controlling bacterial wilt. Seed priming with antagonist microorganisms (Bio-priming) can control seed- and soil born wilt. Therefore, this study was aimed to investigate the effects of tomato seed priming with B. subtilis on seed germination and seedling survival in greenhouses with bacterial wilt outbreak. Completely randomized design of 4 replications was conducted. Results found that tomato seeds priming with B. subtilis gave standard germination (98.00%-100.00%), mean germination time (4.08-4.18 days) and field emergence (96.00%-97.00%) were not statistically different. The survival percentage of tomato seedlings after transplanting in the area with bacterial wilt at the second week shown non statistically different. In week 4 and 6 found that percentage of seedling survival of T1 T2 and T5 were decreased, except T3 and T4 which had the highest of seedling survival at 100% with significant statistical difference. This study could be concluded that tomato seed priming with water for 24 hours and B. subtilis for 12 hours gave seedlings could grow in greenhouses with an outbreak of bacterial wilt. However, this study is only preliminary seed germination and seedling survival testing. It is also necessary to further study seed priming on controlling plant diseases.
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This article is published under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0), which allows others to share the article with proper attribution to the authors and prohibits commercial use or modification. For any other reuse or republication, permission from the journal and the authors is required.References
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