Effects of Buserelin (Suprefact E) Hormone on Seminal Vesicle Physical Characteristics, Gonadosomatic Index and Semen Volume in Male Catfish (Clarias gariepinus)

Abstract

This research aims to study the efficacy of the Buserelin (Suprefact E) hormone on the physical characteristics of the reproductive system in male African catfish. The experiment was conducted on mature male African catfish, which were divided into 3 experimental groups: 1) No hormone injection, 2) Injection of the synthetic hormone Suprefact E without a synergist, and 3) Injection of the synthetic hormone Suprefact E with a synergist. The hormone was injected intramuscularly, and the results were monitored over a period of 12-15 hours. The results revealed that the average Gonadosomatic Index (GSI) values were 0.54 ± 0.14^a, 0.51 ± 0.15, and 0.92 ± 0.22 %, respectively. It was found that experimental group 3 showed a highly significant statistical difference compared to groups 1 and 2 (P<0.01). The study of the physical characteristics of the seminal vesicles showed that the color and appearance in experimental group 1 were predominantly clear white and slender. Group 2 was predominantly clear white and long-slender. Group 3 was predominantly cloudy white and plump. The average lengths of the seminal vesicles were 5.35 ± 0.50, 6.03 ± 1.10, and 6.21 ± 0.77 centimeters, respectively. There was no statistically significant difference among the 3 experimental groups (P>0.05). Additionally, the study on the physical characteristics of the semen revealed average semen volumes of 1.53 ± 0.50, 1.81 ± 0.57, and 3.21 ± 0.35 cc, respectively. Experimental group 3 showed a highly significant statistical difference compared to groups 1 and 2 (P<0.01). Regarding semen color and quantity, the third treatment showed significantly different in both aspects with milky and creamy color and highest quantity of semen which indicate that the semen is matured whereas the others treatment showed relatively clear and small amount of semen. The findings of this study demonstrate that the application of the Buserelin (Suprefact E) hormone in male African catfish is viable, which could serve as an important approach to enhancing commercial breeding efficiency in the future.