Application of Pandora neoaphidis for the control of aphid pests in vegetable crops in Thailand
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Abstract
The entomopathogenic fungus Pandora neoaphidis has great potential for use in the biological control of aphids. In the present study, aphid cadavers were collected from three provinces in Northern Thailand: Chiang Rai, Chiang Mai, and Lamphun. Pathogenicity of three aphid species, Myzus persicae, Lipaphis erysimi, and Aphis fabae, was evaluated in the laboratory. The pure culture of PAN04 showed the highest pathogenicity for the respective aphid species, with average of 95.56% ± 3.25%, 91.83% ± 2.18%, and 92.36% ± 2.73%, respectively. Additionally, the lethal concentration (LC50) based on laboratory tests was calculated for each aphid species, which were 75.55, 78.44, and 59.78 spores/mm2, respectively. Under greenhouse conditions, P. neoaphidis suppressed aphid populations with a percent cumulative mortality (PCM) of 50.94% ± 16.55%, 45.17% ± 4.27%, and 47.36% ± 11.56%, while the mean time to death (MTD) was 5.67 ± 1.15, 5.56 ± 1.53, and 3.67 ± 0.58 days, respectively. In field conditions, the PCM of the respective aphid species averaged 45.00% ± 80%, 34.21% ± 15.3%, and 34.82% ± 7.24%, while the MTD was 6.00 ± 2.00, 6.67 ± 0.58, and 7.42 ± 0.58 days. According to the present study, P. neoaphidis has great potential for controlling economically important aphid species in Thailand.
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References
Abbott, W. S. (1925). A method of computing the effectiveness of insecticides. Journal of Economic Entomology, 18:265-267.
Chen, B., Li, Z. Y. and Feng, M. G. (2007). Occurrence of entomopathogenic fungi in migratory alate aphids in Yunnan Province of China. BioControl, 53:317-326.
Department of Agriculture (2011). Recommendations for preventing and eliminating insects and pests in 2010. Division of Enotomogy and zoology, Department of Agriculture, Bangkok.
DeBach, P. and Rosen, D. (1991). Biological control by natural enemies. Cambridge Cambirdge, UK: Uambridge University Press, pp.440.
Dent, D. R. and Walton, M. P. (1997), Methods in ecological and agricultural entomology. Wallingford, Oxon, UK: CABI Publishing, pp.400.
David, A. S., Matthew, B., Thomus, Y., Simon, B. Y., Pellz, J. K., Pugh, C. and Fellowes, M. D. E. (2003). Genotype and temperature influence pea aphid resistant to a fungal entomopathogen. Physiological Entomology, 28:75-81.
Elkassabany, N. M., Steinkraus, D. C., McLeod, P. J., Correll, P. J. and Morelock, T. E. (1992). Pandora neoaphidis (Entomophthorales: Entomophthoraceae): a potential biological control agent against Myzus persicae (Homoptera: Aphididae) on spinach. Journal of the Kansas Entomological Society, 65:196-199.
Finney, D. J. (1971) Probit Analysis. Cambridge University Press, Cambridge, pp.333.
Rungkiat K. and Samaporn S. (2014). Evaluation of genetic variation and virulence at molecular level of Pandora (Erynia) neoaphidis (Entomophthorales: Entomophthoraceae) used for sustainable control of aphids infested cruciferous crops (Extended phase), unpublished report for National Research Council of Thailand. Rep. Thailand, Bangkok, pp.76.
McLeod, P. J., Steinkraus, D. C., Correll, J. C. and Morelock, T. E. (1998). Prevalence of Erynia neoaphidis (Entomophthorales: Entomopthoraceae) infection of green peach aphid (Homoptera: Aphididae) on spinach in the Arkansas River Valley. Environmental Entomology, 27:796-800.
Owain, R. E., Franzmann, B., Thackray, D. and Micic, S. (2008). Insecticide resistance and implications for future aphid management in Australian grains and pastures: a review. Australian Journal of Experimental Agriculture, 48:1523-1530.
Poinar, G. O. Jr. and Thomas, G. M. (1984). Laboratory guide to insect pathogens and parasites. New York, ny: Plenum Press, pp.392.
Pell, J. K., Eilenberg, J., Hajek, A. E. and Steinkraus, D. C. (2001). Biology, ecology, and pest management potential of Entomophthorales. In: Butt, T.M., Jackson, C., Magan, N. (eds.), Fungal Biological Control Agents: Progress, Problems and Potential. Wallingford, Oxon, UK: CABI Publishing, pp.390.
Stufkens, M.A.W., Walker, G.P. and Martin, N.A. (2005). Lettuce aphid resistance management and prevention strategy. In N. A. Martin and B. P. North. Pesticide Resistance: Prevention and Management Strategies 2005, pp.115-137. Hastings, New Zealand: New Zealand Plant Protection Society Inc.
Surendra K. D. and Semtner, P. J. (2006). Within-Plant Distribution of Pandora neoaphidis (Zygomycetes: Entomophthorales) in populations of the tobacco-feeding form of Myzus persicae (Homoptera: Aphididae) on flue-cured tobacco. Journal of Agricultural and Urban Entomology, 23:65-76.
Sheng, J. C. (2010). Isolation and identification of Pandora neoaphidis and its pathogenicity against turnip aphid (Lipaphis erysimi). Plant Diseases and Pests, 1:43-45.
Tymon, A. M., Shah, P. A. and Pell, J. K. (2004). PCR-based molecular discrimination of Pandora neoaphidis isolates from related entomopathogenic fungi and development of species-specific diagnostic primers. Mycological Research, 108:419-433.
Wilding, N. and Brady, B. L. (1984). Description of pathogenic fungi and bacteria, No. 815. Aberystwyth, uk: Commonwealth Mycological Institute. The Cambrian News.
Wilding, N., Mardell, S. K., Brobyn, P. J., Wratten, S. D. and Lomas, J. (1990). The effect of introducing the aphid-pathogenic fungus Erynia neoaphidis into populations of cereal aphids. Annals of Applied, 117:683-691.
Xu, Q. and Feng, M. (2001). Biomass, sporulation and aphid-infecting virulence of Pandora neoaphidis mycelia produced in repeated liquid culture. Retrived from. https://www.ncbi.nlm.nih.gov/pubmed/12549095
Yeo, H., Pell, J. K., Alderson, P. G., Clark, S. J. and Pye, B. J. (2003). Laboratory evaluation of temperature effects on the germination and growth of entomopathogenic fungi and on their pathogenicity to two aphid species. Pest Management Science, 59:156-165.
