Modelling ozone-based process for decontamination of Opisthorchis viverrini in cyprinid fish
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Abstract
Result indicated that the quadratic model with a correlation coefficient (R²) of 0.9376 was significantly predicted the responses (p<0.05). RSM model showed that optimal condition refers to 0.50 ppm ozone for 20 min, resulting in 100% decontamination of Opisthorchis viverrini metacercariae (OVMC). These findings demonstrated the high efficiency and practical applicability of ozone-based treatment as a food safety intervention to reduce opisthorchiasis transmission through cyprinid fish, with potential impact on public health in endemic regions.
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References
Alexander, J., Knopp, G., Dötsch, A., Wieland, A. and Schwartz, T. (2016). Ozone treatment of conditioned wastewater selects antibiotic resistance genes, opportunistic bacteria, and induce strong population shifts. Science of The Total Environment, 559:103-112.
Alwi, N. A. and Ali, A. (2014). Reduction of Escherichia coli O157, Listeria monocytogenes and Salmonella enterica sv. Typhimurium populations on fresh-cut bell pepper using gaseous ozone. Food Control, 46:304-311.
Baumann, A. A., Myers, A. K., Khajeh-Kazerooni, N., Rosenthal, B., Jenkins, M., O’Brien, C., Fuller, L., Morgan, M. and Lenaghan, S. C. (2024). Aqueous Ozone Exposure Inhibits Sporulation in the Cyclospora cayetanensis Surrogate Eimeria acervulina. Journal of Food Protection, 87:100260.
Botta, C., Ferrocino, I., Cavallero, M. C., Riva, S., Giordano, M., and Cocolin, L. (2018). Potentially active spoilage bacteria community during the storage of vacuum packaged beefsteaks treated with aqueous ozone and electrolyzed water. International Journal of Food Microbiology, 266:337-345.
Chen, J., Lan, X., Jia, R., Hu, L. and Wang, Y. (2022). Response Surface Methodology (RSM) Mediated Optimization of Medium Components for Mycelial Growth and Metabolites Production of Streptomyces alfalfae XN-04. Microorganisms, 10:1854.
Epelle, E. I., Macfarlane, A., Cusack, M., Burns, A., Okolie, J. A., Mackay, W., Rateb, M. and Yaseen, M. (2023). Ozone application in different industries: A review of recent developments. Chemical Engineering Journal, 454:140188.
Guzel-Seydim, Z. B., Greene, A. K. and Seydim, A. C. (2004). Use of ozone in the food industry. LWT - Food Science and Technology, 37:453-460.
Hazbawi, I. and Safaeinezhad, M. (2023). Optimizing the Effect of Spike Density and Combine Speed for Reducing Wheat Loss Using Response Surface Methodology. Biomechanism and Bioenergy Research, 2:19-27.
Kaewpitoon, N., Kaewpitoon, S. J. and Pengsaa, P. (2008). Opisthorchiasis in Thailand: Review and current status. World Journal of Gastroenterology, 14:2297-2302.
Khalifa, A., Temsahy, M. and Abou El Naga, I. (2001). Effect of ozone on the viability of some protozoa in drinking water. Journal of the Egyptian Society of Parasitology, 31:603-616.
Owolabi, R. U., Usman, M. A. and Kehinde, A. J. (2018). Modelling and optimization of process variables for the solution polymerization of styrene using response surface methodology. Journal of King Saud University - Engineering Sciences, 30:22-30.
Pinlaor, S., Onsurathum, S., Boonmars, T., Pinlaor, P., Hongsrichan, N., Chaidee, A., Haonon, O., Limviroj, W., Tesana, S., Kaewkes, S. and Sithithaworn, P. (2013). Distribution and Abundance of Opisthorchis viverrini Metacercariae in Cyprinid Fish in Northeastern Thailand. The Korean Journal of Parasitology, 51:703-710.
Rajabi, O., Sazgarnia, A., Abbasi, F. and Layegh, P. (2015). The activity of ozonated olive oil against Leishmania major promastigotes. Iranian Journal of Basic Medical Sciences, 18:915-919.
Roy, S., Choudhury, B., Johnson, J. and Schindler-Tyka, A. (2021). Application of dielectric barrier discharge for improving food shelf life and reducing spoilage. Scientific Reports, 11:1-9.
Sithithaworn, P., Pipitgool, V., Srisawangwong, T., Elkins, D. B. and Haswell-Elkins, M. R. (1997). Seasonal variation of Opisthorchis viverrini infection in cyprinoid fish in north-east Thailand: Implications for parasite control and food safety. Bulletin of the World Health Organization, 75:125-131.
Sripong, K., Uthairatanakij, A. and Jitareerat, P. (2022). Impact of gaseous ozone on microbial contamination and quality of fresh-cut durian. Scientia Horticulturae, 294:110799.
Xue, W., Macleod, J. and Blaxland, J. (2023). The Use of Ozone Technology to Control Microorganism Growth, Enhance Food Safety and Extend Shelf Life: A Promising Food Decontamination Technology. Foods, 12:814.