Efficiency of Bacillus subtilis in inhibiting the mycelial growth of Ganoderma boninense the causal agent of basal stem rot disease in oil palm (Elaeis guineensis Jacq.)
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Abstract
This research evaluated the efficacy of biological products derived from Bacillus subtilis subsp. subtilis RUTs001, Bacillus subtilis RUTs002 and Bacillus subtilis RUTs003 in inhibiting the mycelial growth of Ganoderma boninense, the causal agent of basal stem rot disease in oil palm (Elaeis guineensis) on agar media and on mushroom bag. The results demonstrated that the combinations of B. subtilis subsp. subtilis RUTs001 + B. subtilis RUTs003 and B. subtilis subsp. subtilis RUTs001 + B. subtilis RUTs002 emerged as the most effective, inhibiting the mycelial growth of G. boninense and resulting in a notable slowdown of mycelial growth. The time required for full colonization of the substrate was recorded at 35 days after inoculation. Followed by commercial B. subtilis product achieved full colonization at 34 days. Moreover, the use of bioproduct of B. subtilis can degrade mycelium completely within 150 days of its colonization. While in the control, mycelium growth was slower, and full colonized was achieved at 32 days.
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References
Ahmad, T., Xing, F., Nie, C., Cao, C., Xiao, Y., Yu, X., Moosa, A. and Liu, Y. (2023). Biocontrol potential of lipopeptides produced by the novel Bacillus subtilis strain Y17B against postharvest Alternaria fruit rot of cherry. Frontiers in Microbiology, 14:1150217. Retrieved from http://doi: 10.3389/fmicb.2023.1150217
Alexander, A. and Chong, K. P. (2014). Combination of biological agents in suppressing colonization of Ganoderma boninense of basal stem rot. American-Eurasian Journal of Sustainable Agriculture, 8:1-7.
Alexander, A., Abdullah, S., Rossal., S. and Chong, K. P. (2017). Evaluation of the efficacy and mode of action of biological control for suppression of Ganoderma boninense in oil palm. Pakistan Journal of Botany, 49:1193-1199.
Arsyad, M., Amiruddin, A., Suharno, S. and Jahroh, S. (2020). Competitiveness of palm oil products in international trade: an analysis between Indonesia and Malaysia. Journal of Sustainable Agriculture, 35:157-167.
Aydogdu, M., Sulu, S. M., Kurbetli, L. and Sulu, G. (2021). In vitro and in vivo biological control of the green mold using different bacteria in button mushroom cultivation. Egyptian Journal of Biological Pest Control, 31. Retrieved from http://doi:10.1186/s41938-021-00401-w
Bharudin, I., Ab Wahab, A. F. F., Smad, M. A. A., Yie, N. X., Zairun, M. A., Bakar, F. D. A. and Murad, A. M. A. (2022). Review update on the life cycle, plant-microbe interaction, genomics detection and control strategies of the oil palm pathogen Ganoderma boninense. Biology, 11:251. Retrieved from http://doi.org/10.3390/ biology11020251
Bivi, M. S., Paiko, A. S., Khairulmazmi, A., Akhtar, M. S. and Idris, A. S. (2016). Control of basal stem rot disease in oil palm by supplementation of calcium, copper, and salicylic acid. Plant Pathology Journal, 32:396-406.
Haw, Y. H., Lai, K.W., Chuah, J. H., Bejo, S. K., Husin, N. A., Hum, Y. C., Yee, P. L., Tee, C. A. T. H., Ye, X. and Wu, X. (2023). Classification of basal stem rot using deep learning: a review of digital data collection and palm disease classification methods. Computer Sciences, 9:1325. Retrieved from http://doi:10. 7717/ peerj-cs.1325
Khoo, Y, W. and Chong, K. P. (2023). Ganoderma boninense: general characteristics of pathogenicity and methods of control. Frontiers in Plant Science, 14:1156869. Retrieved from http://doi:10.3389/ fpls.2023. 1156869
Kumar, N. P. and Krishna, C. (2023). A Multi-Criteria Assessment of the Impact of Previous Land Use and Current Management Practices on the Performance of Oil Palm on Smallholders’ Plots in Southern Thailand (Master Thesis). Norwegian University of Life Sciences.
Lim, P., Gansau, J. and Chong, K. (2019). Biocontrol of basal stem rot pathogen Ganoderma Boninense by Pseudomonas auruginosa. Bangladesh Journal Botany, 48:209-215.
Maherani, V. F. A., Mubarik, N. R., Priyatno, J. A. and Putra, I. P. (2024). Biosurfactant activity of Bacillus strain LP04 isolate and its antifungal potency against Ganoderma boninese and Fusarium sp. Hayati Journal Bioscience, 31:725-732.
Nagappan, J. (2023). Genome Sequencing and Analysis of G. boninense, G. zonatum, G. miniatocinctum, and G. tornatum Towards Understanding Basal Stem Rot of Oil Palm (Doctoral Dissertation). University of Nottingham.
Nuchaikaew, W., Vanichpakorn, P. and Seephueak, P. (2024). The efficacy of bioproduct of Bacillusspp. on mycelium growth and controlling green mold disease of Ganoderma lucidum. Songklanakarin Journal of Science and Technology, 46:499-506.
Nur Azura, A. B., Yusoff, M., Tan, G. Y. A., Jegadeesh, R., Appleton, D. R. and Vikineswary, S. (2016). Streptomyces sanglieri which colonised andenhanced the growth of Elaeis guineensis Jacq. seedlings was antagonistic to Ganoderma boninensein in vitro studies. Journal Industrial Microbiology and Biotechnology, 43:485-493.
Nusaibah, S. A., Saad, G. and Tan, G. H. (2017). Antagonistic efficacy of Trichoderma harzianum and Bacillus cereus against Ganoderma disease of oil palm via dip, place and drench (DPD) artificial inoculation technique. International Journal of Agriculture and Biology, 19:299-306.
Puspita, F., Hadiwiyono, Poromorto, S. H. and Roslim, D. I. (2019). The application of different Bacillus subtilis contained formula as bio fungicide tablet to control Ganoderma boninense in oil palm nurseries. IOP Conference Series: Earth and Environmental Science. doi:10.1088/1755-1315/250/1/012052
Puspita, F., Hadiwiyono, Poromorto, S. H. and Roslim, D. I. (2020). Induced resistance by Bacillussubtilis on oil palm seedling infected by Ganoderma boninense. Biodiversitas, 21:28-33.
Rajendran, L., Karthikeyan, G., Raguchander, T. and Samiyappan, R. (2018). Effect of Endophytic bacteria Bacillus subtilis EPC 5 on basal stem rot disease spread and nut yield in coconut plantations. Journal of Applied Horticulture, 20:207-212.
Rakib, M. R. M., Bong, C. F. J., Khairulmazmi, A., Idris, A. S., Jalloh, M. B. and Wahida, N. H. (2017). Ganoderma species of basal and upper stem rots in oil palm (Elaeis guineensis) in Sarawak, Malaysia. Journal of Academia UiTM Negeri Sembilan, 5:27-35.
Rashad, Y. M. and Moussa, T. A. (2020). Biocontrol agents for fungal plant diseases management. In: Saleh, M., Abu-Hashim, M. and El-Wakeil, N. eds. Cottage industry of biocontrol agents and their applications: Practical aspects to deal biologically with pests and stresses facing strategic crops, Berlin, Springer Verlag, pp.337-363.
Rupaedah, B., Prasetyo, A. E., Hidayat, F., Asiani, N., Wahid, A., Nurlaila. and Lutfia, A. (2024). Evaluation of microbial biocontrol agent for Ganoderma boninense management in oil palm nurseries. Agricultural Sciences, 23:236-244.
Saeyang, R. and Nissapa, A. (2021). Trade competitiveness in the global market: an analysis off our palm oil products from Indonesia, Malaysia and Thailand. International Journal of Agricultural Technology, 17:1077-1094.
Sapak, Z., Meon, S. and Ahmad, Z. A. M. (2008) Effect of endophytic bacteria on growth and suppression of Ganoderma infection in oil palm. International Journal of Agriculture and Biology, 10:127-132.
Shigetomi, Y., Ishimura, Y. and Yamamoto, Y. (2020). Trends in global dependency on the Indonesian palm oil and resultant environmental impacts. Scientific Reports, 10:20624. Retrieved from http://doi.org/10.1038/s4159 8-020-77458-4
Sitepu, A., Yenni, Y. and Sujadi. (2022). Parental selection based on breeding value of dura x tenera bunch component. Jurnal Penelitian Kelapa Sawit, 30:15-26.
Supramani, S., Rejab, N. A., Ilham, Z., Wan-Mohtar, W. A. A. Q. I. and Ghosh, S. (2022). Basal stem rot of oil palm incited by Ganoderma species: a review. European Journal of Plant Pathology, 1-20. Retrieved from http://doi: 10.1007/s10658-022-02546-2
Walderich, A. (2024). Palm oil industry in Thailand. Retrieved from https://eos.com/blog/palm-oil-malaysia-indonesia-thailand/
Widiantini, F., Nugraha, G. T., Yulia, E. and Nasahi, C. (2024). Antagonistic effects of bacterial rhizosphere of oil palm in biocontrol of basal stem rot disease (Ganoderma boninense Pat.). Hayati Journal of Biosciences, 31:1071-1081.
Zakaria, L. (2023). Basal stem rot of oil palm: the pathogen, disease incidence, and controlmethods. Plant Disease, 107:603-615.
Zulfikar, A., Nur Layla, I., Preecha, C., Seephueak, W. and Seephueak, P. (2018). Use of Antagonistic bacteria from spent mushroom compost for controlling damping-off cause by Fusarium solani in tomato. Proceedings of the 6th Asian Academic Society International Conference (AASIC): A Trans Formative Community: Asia in Dynamism, Innovation, and Globalization, Mae Fah Luang University, Thailand, 6:630-638.
