Production, partial purification and immobilization of alpha amylase using new bacterial isolate and its nanoform application for modification of wool fibers dyed with madder natural dye
Article Sidebar
Main Article Content
Abstract
The amylase producing bacterial isolates E coli NRC1169 showed maximum amylase production and selected for physiological and biochemical reactions. Fermentation process on suitable medium showed the proper producing isolates. Partial purification of the crude enzyme, its characterization and using nano-chitosan as carrier were recorded. Partially purified enzyme was investigated in the field of textile for pre-treatment of wool fibres dyed with madder natural dye. The maximum activity resulted in using fermentation medium consisted of starch ,10 peptone 10 yeast extract 5, ammonium sulphate 5, magnesium sulphate 0.25, CaCl 0.25 tween 80 1ml at pH 5.6, fermentation time 48 h at 30C0. The obtained amylase was undergoing partial purification using ammonium sulphate at concentration of 60%. The purified enzyme was applied to textile industries. Result indicated that the wool fibres samples treated with enzyme free and immobilised exhibited higher colour strength and fastness properties than the untreated fibres. The antimicrobial activity were tested using E. coli, B. subtilus, C. albicans, S. aureus for the fibres treated with amylase gave higher inhibition than the untreated fibres
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Adeel, S., Rehman, F. U., Rafi, S., Zia, K. M. and Zuber, M. (2019). Environmentally Friendly Plant-Based Natural Dyes: Extraction, Methodology and Applications. Plant and Human Health, 2:383-415.
Ali, N. F. and Abd- Elsalam, I. S. (2020). Antimicrobial characteristics of wool fibers treated with chitosan-propolis nano composite and dyed with natural dye extracted from Red Prickly Pear. International Journal of Agricultural Technology, 16:223-236.
Ali, N. F. and Abd-Elsalam, I. S. (2022). Improvement of wool dyeing quality and its antimicrobial activity using nano forms of silver. International Journal of Agricultural Technology, 18:437-446.
Asif, A. H., A. K.M. and Hasan, M. Z. (2018). Application of nanotechnology in modern Textile: A Review. International Journal of Current Engineering and Technology online.8, 2.
Atalla, S. M. M., Gamal, N. G. El., b, Awad, H. M. and Ali, N. F. (2019). Production of pectin lyase from agricultural wastes by isolated marine, Penicillium expansum RSW_SEP1 as dye wool fiberc. Heliyon, 5:e02302.
Chequer, F. M. D., Oliveira, G. A. R., Ferraz, E. R. A., Cardoso, J. C., Zanoni, M. V. B. and de Oliveira, D. P. (2013). Textile dyes: Dyeing process and environmental impact. In Eco-Friendly Textile Dyeing and Finishing; Gunay, M., Ed.; IntechOpen: London, UK, pp. 151-176.
Gahlot, M., Fatima, N. and Papnai, N. (2008). Jatropha flowers; a natural colourant for dyeing of cotton, wool and silk. Colourage, 52:96-200.
George, R. and Georrge, J. J. (2020). Thermostable alpha-amylase and its activity, stability and industrial relevance studies. Proceedings of the National Conference on Innovations in Biological Sciences (NCIBS) 2020, 12 p.
Gupta, R., Paresh, G., Mohapatra, H., Goswami, V. and Chauhan, B. (2003). Microbial α- amylases: a biotechnological perspective. Process Biochemistry, 38:1599-1616.
Malacara, D. (2002). Color vision and colorimetry: Theory and applications. Color Research and Application, 28:77-78.
Mojsov, K. (2011). Application of enzymes in the textile industry: A review. Conference: II International Congress "Engineering, Ecology and Materials in the Processing Industry", 09-11 March 2011, pp.230-239.
Oyeleke, S. B., Auta, S. H. and Egwim, E. (2010). Production and characterization of amylase produced by Bacillus megaterium isolated from a local yam peel dumpsite in Minna, Niger State. Journal of Microbiology and Antimicrobials, 2:88-92.
Pokhrel (2013). Adolescent Neurocognitive Development, Self-Regulation, and School-Based Drug Use Prevention. Prevention Science, 14(3), DOI:10.1007/s11121-012-0345-7
Samanta, A. K., Agarwal, P., Konar, A. and Datta, S. (2008). Characterisation and application of purified selective natural dyes on jute. International Dyer, 192:25-32.
Shahmoradi Ghaheh, F., Mortazavi, S. M., Alihosseini, F., Fassihi, A., Shams Nateri, A. and Abedi, D. (2014). Assessment of antibacterial activity of wool fabrics dyed with natural dyes. Journal of Cleaner Production, 72:132-145.
van der Maarel, M. J. E. C., van der Veen, B., Uitdehaag, J. C. M., Leemhuis, H. and Dijkhuizen, L. (2002). Properties and applications of starch-converting enzymes of the α-amylase family. Journal of Biotechnology, 94:137-155.
Xue, Z. and Jin-xin, H. (2011). Improvement in dyeability of wool fabric by microwave treatment. Indian Journal of Fibre and Textile Research, 36:58-62.
Zhan, Y. Z. and Zhao, X. (2009). Application of microwave technology in textile dyeing and finishing. Text. Aux, 7.
