Isolation and in vitro control of a fungus infecting common beans in the Darjeeling hills
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Abstract
From the results of the agar cup bioassay, it was evident that among six plant materials tested, only four extracts were effective against the pathogen, Aspergillus sp. The four potential plants were Bergenia ciliate, Artemisia vulgaris, Centella asiatica, and Urtica dioica. Among these four effective plant materials, Bergenia ciliate had the most inhibitory effect, which developed an inhibition zone with a diameter of 12.28 mm. Next to Bergenia ciliate, there was inhibitory activity in Artemisia vulgaris leaf extract, which showed an inhibition zone of 10 mm in diameter. The inhibitory effect of these botanicals against the fungus was verified by the poisoned food technique. The medium of growth was supplemented with aqueous extracts of the plant materials. Final concentrations of the plant extracts in the medium were maintained at 5 %, 10 % and 20 %. The inhibition of growth was computed in per cent. Data were recorded after 5 and 10 days. The inhibitory effect of Bergenia ciliate and Artemisia vulgaris was significantly high and 100% inhibition was by 10 % and 20% concentrations of the aqueous extracts after 10 days of incubation. Thus, the present study significantly sheds light on the management of the disease of common bean plants caused by Aspergillus sp.
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