Duration for callus propagation of indica rice (Oryza sativa L.) cultivar Sangyod in suspension culture
Article Sidebar
Main Article Content
Abstract
Calli of indica rice (Oryza sativa L.) cultivar Sangyod were propagated in a liquid culture medium containing 4.10 gL-1 modified Chu/Gamborg (NB) basal medium, 1 gL-1 L-proline, 2 mgL-1 2,4-dichlorophenoxy acetic acid (2,4-D), 1 mgL-1 1-naphthalene acetic acid (NAA) and 30 gL-1 sucrose, maintained in shaker flasks (25±2°C, 100 rpm, under light for 27 days. Day 12 was found to be the best time for callus propagation, indicated by fresh mass (F.M.) doubling time (td) 11.73 day-1 and the highest cell density up to 66.83 gL-1 F.M. and 4.98 gL-1 dry mass, in the exponential phase. The productivity was 2.61 gL-1day-1 F.M. and yield of cell mass (Yx/s) was 1.08 gcellg-1glucose F.M. The specific growth rate (μ) was 0.06 day-1 F.M. This data can be used to choose the optimum time for repeated subculturing callus to increase the productivity of high cell density cultures and it is an important for further study the physiological and morphological differentiation of cell suspension culture, improving plant regeneration systems.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Amalraj, V. (2012). Auxins and Cytokinins -and Browning of the Callus. Retrieved from https://www.researchgate.net/post/Auxins_and_cytokinins- and_browning_of_the_callus.
Amarasinghe, A. A. Y. (2009). Effects of copper sulphate and cobalt chloride on in vitro performances of traditional indica rice (Oryza sativa L.) varieties in Sri Lanka. Journal of Agricultural Sciences, 4:132-141.
Antimanon, S. (2015). Process Development of Recombinant Protein Production by Thermotolerant Yeast Ogataea thermomethanolica Using Constitutive OthGAP Promoter. (Master Thesis). Thammasat University, Thailand.
Anderson, H. (2017). Tissue Culture Types, Techniques and Process. Microscopemaster. Retrieved from https://www.microscopemaster.com/tissue-culture.html.
Arnold, M. (1999). Feeding the ten billion: plants and population growth. In: Evans LT ed. Experimental Agriculture, Cambridge, University Press, pp. 507-516.
Din, A. R. J. M., Ahmad, F. I., Wagiran, A., Samad, A. A., Rahmat, Z. and Sarmidi, M. R. (2016). Improvement of efficient in vitro regeneration potential of mature callus induced from Malaysian upland rice seed (Oryza sativa cv. Panderas). Saudi Journal of Biological Sciences, 23:69-77.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. T. and Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28:350-356.
Fankhauser, D. B. (2015). Bacterial Growth Curve. Retrieved from https://krishnapcandra.files. wordpress.com/2011/09/bacterial-growth-curve_-exponential.pdf.
Farjaminezhad, R., Zare, N., Zakaria, R. A. and Farjaminezhad, M. (2013). Establishment and optimization of cell growth in suspension culture of Papaver bracteatum: a biotechnology approach for thebaine production. Turkish Journal of Biology, 37:689-697.
Godoy-Hernández, G. and Vázquez-Flota, F. A. (2006). Growth measurements: estimation of cell division and cell expansion. In: Loyola-Vargas VM and Vazquez-Flota FA ed. Plant Cell Culture Protocols, USA, Humana Press, pp.51-58.
Hussein, S., Halmi, M. I. E. and Kiong, A. L. P. (2016). Modelling the growth kinetics of callus cultures from the seedling of Jatropha curcas L. according to the modified Gompertz model. Journal of Biochemistry, Microbiology and Biotechnology, 4:20-23.
International Rice Research Institute (2013). Rice almanac, 4th ed. Global Rice Science Partnership, Philippines, pp.283.
Linh, H. T. (2017). In Vitro Regeneration of Indica Rice (Oryza sativa L.) cultivar Sangyod and Its Transformation by Agrobacterium tumefaciens. (Master Thesis). Prince of Songkla University, Thailand.
Meesook, K., Pongtongkam, P., Pongjaroenkit, S. and Poeaim, A. (2019). Callus induction and effect of gamma ray on rice callus (Oryza sativa L.) cultivar Sangyod Phatthalung. 21st National Genetics Conference 2019, Chonburi, Thailand, pp.137-145.
Poeiam, A. and Saengdeuan, N. (2000). Callus formation and cell suspension in Leuang Pratew 123 rice (Oryza sativa L.). Proceedings of the 38th Kasetsart University Annual Conference: Fisheries and Science, Bangkok, Thailand, pp.472-479.
Poraha, R., Poeaim, A., Pongjaroenkit, S. and Pongthongkam, P. (2015). Callus induction and growing cell suspension culture of jow haw rice (Oryza sativa L.). Journal of Agricultural Technology, 11:297-305.
Poraha, R. (2016). Callus and cell suspension induction and regeneration of rice (Oryza sativa L.) by tissue culture. (Master Thesis). King Mongkut's Institute of Technology Ladkrabang, Thailand.
Quadram Institute Bioscience (2008). Bacterial Growth. Retrieved from http://www.ifr.ac.uk/ bacanova/project_backg.html.
Song, J. (2003). Sustaining food security. Rice science: Innovations and impact for livelihood. International Rice Research Conference, Beijing, China, pp.5-7.
Srinuttrakul, W., Permnamtip, V. and Yoshida, S. (2018). Heavy metals in Sangyod rice samples cultivated in Phatthalung, Thailand. Food and Applied Bioscience Journal, 6:45-54.
Srisawat, U., Panunto, W., Kaendee, N., Tanuchit, S., Itharat, A., Lerdvuthisopon, N. and Hansakul, P. (2010). Determination of phenolic compounds, flavonoids, andantioxidant activities in water extracts of Thai red and white rice cultivars. Journal of the Medical Association of Thailand, 93:83-91.
Sundram, T. C., Annuar, M. S. M. and Khalid, N. (2012). Optimization of culture condition for callus induction from shoot buds for establishment of rapid growing cell suspension cultures of mango ginger (Curcuma mangga). Australian Journal of Crop Science, 6:1139.
Trejo-Tapia, G., Amaya, U. M., Morales, G. S., Sánchez, A. D. J., Bonfil, B. M., Rodríguez-Monroy, M. and Jiménez-Aparicio, A. (2002). The effects of cold-pretreatment, auxins and carbon source on another culture of rice. Journal of Plant Biotechnology, 71:41-46.