Investigation of blast resistance genes in a broad-spectrum resistant indigenous rice, Yang Mawng variety

Main Article Content

Suksiri, W.
Parinthawong, N.

Abstract

The indigenous rice Yang Mawng variety was highly resistant to 24 isolates and moderate resistant to 3 isolates of P. oryzae. The results confirmed the broad-spectrum resistance to blast disease of Yang Mawng variety. The pathogenicity test of 27 isolates of P. oryzae was conducted on 31 Near Isogenic Lines (NILs). The result showed that virulence index (VI) was valued from 0 to 0.50 and the most pathogenic isolate on NILs was UBN2010 13515. The investigation for 8 rice blast disease resistance genes of Yang Mawng variety was done using blast resistance gene specific primers and PCR technique. The result showed that 5 blast resistance genes including Pi9, Pigm(t), Pi54, Pib and Pita, were detectable. The information of this research is a preliminary data for study of resistance genes present in Yang Mawng variety.

Article Details

How to Cite
Suksiri, W., & Parinthawong, N. (2020). Investigation of blast resistance genes in a broad-spectrum resistant indigenous rice, Yang Mawng variety. International Journal of Agricultural Technology, 16(1), 155–162. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6482
Section
Original Study

References

Ashkani, S., Rafii, M. Y., Shabanimofrad, M., Miah G., Sahebi M., Azizi, P. and Nasehi, A. (2015). Molecular breeding strategy and challenges towards improvement of blast disease resistance in rice crop. Frontiers in plant science, 6:886.

Cho, Y. C., Kwon, S. W., Choi, I. S., Lee, S. K., Jeon, J. S., Oh, M. K. and Kim, Y. G. (2007). Identification of major blast resistance genes in Korean rice varieties (Oryza sativa L.) using molecular markers. Journal of Crop Science and Biotechnology, 10:265-276.

Das, A., Soubam, D., Singh, P. K., Thakur, S., Singh, N. K. and Sharma, T. R. (2012). A novel blast resistance gene, Pi54rh cloned from wild species of rice, Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae. Functional & integrative genomics, 12:215-228.

Deng, Y., Zhu, X., Shen, Y. and He, Z. (2006). Genetic characterization and fine mapping of the blast resistance locus Pigm(t) tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety. Theoretical and Applied Genetics, 113:705-713.

Devanna, N. B., Vijayan, J. and Sharma, T. R. (2014). The blast resistance gene Pi54 of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains. PloS one, 9:104840.

He, X., Liu, X., Wang, L., Lin, F., Cheng, Y. and Pan, Q. (2012). Identification of the novel recessive gene pi55(t) conferring resistance to Magnaporthe oryzae. Science China Life Sciences, 55:141-149.

Jia, Y., Wang, Z. and Singh, P. (2002). Development of dominant rice blast Pi-ta resistance gene markers. Crop Science, 42:2145-2149.

Kladmook, M., Sangduen, N. and Hongtrakul, V. (2009). Investigation of blast disease resistant genes in Thai local rice. Genomics and Genetics, 2:36-43.

Liu, G., Lu, G., Zen, L. and Wang, G. L. (2002). Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6. Molecular Genetics and Genomics, 267:472-480.

Phaitreejit, K., Srikaew, E. O., Jantasuriyarat, C., Sriwongchai, T. and Kate-ngam, S. (2011). Screening Thai landrace rice for blast resistance gene Pi9, Pi36, Pigm (t) using DNA markers. Genomics and Genetics, 4:52-62.

Rai, A. K., Kumar, S. P., Gupta, S. K., Gautam, N., Singh, N. K. and Sharma, T. R. (2011). Functional complementation of rice blast resistance gene Pi-kh (Pi54) conferring resistance to diverse strains of Magnaporthe oryzae. Journal of Plant Biochemistry and Biotechnology, 20:55-65.

Ramkumar, G., Srinivasarao, K., Mohan, K. M., Sudarshan, I., Sivaranjani, A. K. P., Gopalakrishna, K. and Rani, N. S. (2011). Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54(Pik h). Molecular breeding, 27:129-135.

Roumen, E., Levy, M. and Notteghem, J. L. (1997). Characterization of the European pathogen population of Magnaporthe grisea by DNA finger printing and pathotype analysis. European Journal of Plant Pathology, 103:363-371.

Salih, A., Sreewongchai, T., Sripichitt, P. and Parinthawong, N. (2013). Identification of blast resistant varieties from landrace, improved and wild species of rice. Kasetsart Journal (Natural Science), 47:1-7.

Singh, W. H., Kapila, R. K., Sharma, T. R. and Rathour, R. (2015). Genetic and physical mapping of a new allele of Pik locus from japonica rice ‘Liziangxintuanheigu’. Euphytica, 205:889-901.

Srivastava, D., Shamim, M. D., Kumar, D., Pandey, P., Khan, N. A. and Singh, K. N. (2014). Morphological and molecular characterization of Pyricularia oryzae causing blast disease in rice (Oryza sativa) from north India. International Journal of Scientific and Research Publications, 4:2250-3153.

Xiao, N., Wu, Y., Pan, C., Yu, L., Chen, Y., Liu, G. and Liang, C. (2017). Improving of rice blast resistances in japonica by pyramiding major R genes. Frontiers in plant science, 7:1918.