Biocontrol potential of Trichoderma viride, Pseudomonas fluorescens for Fusarium wilt of cowpea (Vigna unguiculata L.)
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Abstract
Trichoderma viride (MH333256), Pseudomonas fluorescens (Ps01), Fusarium oxysporum (Fu04) were morphological identified and biochemical characterization was determined under compound microscope. All isolates were phylogenetic confirmed by genetic DNA isolation and PCR analysis. The healthy seeds of Cowpea (Vigna unguiculata L.) were used in experiment. Results described in treatments of control, Ps01, Tr02, Fu04, Ps01+ Fu04, Tr02+ Fu04, Ps01+ Fu04+Tr02, after inoculation to the seeds sown in the pots. In pot experiment, the bio-inoculated plant (Tr02) significantly enhanced the plant biomass, chlorophyll, nitrogen, NPK content and soil microbial population after 45 DAI. Bioinoculant (Tr02, Ps01) highly inhibited plant pathogen (Fu04) and increased in plant fresh weight (13.4 g) when compared to the control (9 g) and Fu04 (6 g).
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