Effect of colchicine on survival rate and ploidy level of hydrid between Dendrobium santana x D. friedericksianum orchid
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Abstract
The effect of colchicine on survival rate and ploidy level of hydrid between Dendrobium santana and D. friedericksianum orchid was studied. In vitro polyploid induction of this hydrid orchid was carried out by soaking nodal explant in different concentrations of colchicine for 24, 48 and 72 hours. The results showed that LD50 of colchicine for 24, 48 and 72 hours was 0. 052%, 0.041% and 0.011%, respectively. The treatment of colchicine at the above concentrations and durations increased DNA content from 33 to 50 % of the original DNA content as analyzed by flow cytometry (FCM) technique. This result was in accordance with physiological characteristics, which revealed the bigger size of guard cells but lower density and increase in number of chloroplasts in guard cell.
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