Comparison of Bioactive Compounds Contents and Antioxidant Activity in Fresh-Dried Garcinia atroviridis and Commercially Available Dried Garcinia Fruits
Keywords:
Garcinia atroviridis fruit, Bioactive compounds, Antioxidant activity, Fresh-dried and commercially available dried garcinia fruitsAbstract
The objective of this study was to compare the contents of bioactive compounds, including total phenolics, flavonoids and tannins, as well as to evaluate the antioxidative activity in fresh-dried Garcinia atroviridis fruits and commercially available dried garcinia fruits. Extracts were prepared by macerating slices of both types of fruits in 95% (v/v) ethanol at a sample-to-solvent ratio of 1:4 (w/v) for 7 days at room temperature. The obtained extracts were filtered and concentrated by evaporating the solvents under reduce pressure using a rotary evaporator. Then, the contents of total phenolics, flavonoids, and tannins were determined by measuring absorbance with UV-visible spectrophotometer. The antioxidative activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay. The results were expressed as the percentage of DPPH radical scavenging activity (%SA) and the 50% inhibitory concentration (IC50). The results showed that the extract of fresh-dried garcinia fruit contained significantly higher amounts of all three bioactive compounds and exhibited higher %SA values than the extract of commercially dried garcinia (p < 0.05). However, the IC50 values of both extracts were found to be comparable, at 1.30 and 1.23 ug/ml, respectively. These findings indicate that. The appropriate manufacturing process of dried Garcinia atroviridis slices affects the content of bioactive compounds and antioxidant properties to scavenge free radicals.
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