Decolorization of Methylene Blue and Malachite Green Using Hydrilla (Hydrilla verticillata)

Jassada   Pangsri; Thanawan PANICH-PAT

Authors

Jassada Pangsri Department of Science and Bioinnovation, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand
Thanawan PANICH-PAT Department of Science and Bioinnovation, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand

Keywords:

Absorbance, Decolorization, Hydrilla verticillata, Malachite green, Methylene blue

Abstract

This study evaluated the decolorization performance of Hydrilla verticillata in water contaminated with methylene blue (MB) and malachite green (MG) at an initial dye concentration of 100 mg/L under continuous fluorescent illumination (24 h). MB exhibited a gradual decrease in absorbance, achieving 87% decolorization after 24 h and 93% after 96 h. MG was decolorized more rapidly, reaching 43% after 1 h and complete (100%) decolorization after 72 h. After 96 h, total Kjeldahl nitrogen (TKN) increased significantly (p < 0.05), whereas dissolved oxygen (DO) decreased significantly (p < 0.05) in both treatments, while pH remained stable (6–7). FT-IR spectra of MB and MG before and after treatment showed similar characteristic absorption bands, with no detectable changes in the major peaks, indicating that no detectable structural changes occurred in the dye molecules during the experimental period.

References

Abdullah T., Al-Fawwaz, & M. Abdullah. (2016). Decolorization of methylene blue and malachite green by immobilized Desmodesmus sp. isolated from North Jordan. International Journal of Environmental Science and Development, 7(2), 95-99.

Abdullah, R.S., Anuar, N., and Hasan, H.A. (2018). Phytotransformation of methylene blue from water using aquatic plant (Azolla pinnata). Journal of Hazadous Materials, 388, 121569.

Ahila, K.G., Ravindran, B., Muthunarayanan, V., Dinh Duc Nguyen, D.D., Nguyen, X.C., Chang, S.W., Nguyen, V.N., & Chandran Thamaraiselvi, C. (2021). Phytoremediation potential of freshwater macrophytes for treating dye-containing wastewater. Sustainability, 13(1), 329. doi.org/10.3390/su13010329.

Anjana, S. & V.S.G. Thanga. (2011). Phytoremediation of synthetic textile dyes. Asian Journal of Microbiology, Biotechnology & Environmental Sciences, 13, 31–34.

Bayram, O., Köksal, E., Göde, F., and Pehlivan, E. (2022). Decolorization of water through removal of methylene blue and malachite green on biodegradable magnetic Bauhinia variagata fruits. The International Journal of Phytoremediation, 24(3), 311-323. doi: 10.1080/15226514.2021.1937931

Chahar, R., Mukherji, R., Chakraborty, S., Mukherji, S.D., & Chauhan, M.S. (2024). Effective removal of toxic dye [Malachite green] by aquatic plant Hydrilla verticillata and Najas minor as low cost adsorbent. Environment, Development and Sustainability. doi.org/10.1007/s10668-024-05406-7.

Chandra, R., Dubey, N.K., & Kumar, V. (2017). Phytoremediation of environmental pollutants. CRC Press, Boca Raton.

Clemmensen, S., Jensen, J.C., Jensen, N.J., Meyer, O., Olsen, P.,& Würtzen., G. (1984). Toxicological studies on malachite green: A triphenylmethane dye. Archives of Toxicology, 56, 43–45.

Coates, J. 2006. Interpretation of Infrared Spectra, A Practical Approach. Wiley online library. https://doi.org/10.1002/9780470027318.a5606

Imron, M.F., Kurniawan, S.B., Soegianto, A.,& Wahyudianto, F.E. (2019). Phytoremediation of methylene blue using duckweed (Lemna minor). Heliyon, 5, e02206.

Karaghool, H.A.K.; Hashim, K.; Kot, P.; Muradov, M. Preliminary Studies of Methylene Blue Remotion from Aqueous Solutions by Ocimum basilicum. Environments 2022, 9, 17. https://doi.org/10.3390/environments9020017

Khataee, A.R., Movafeghi, A., Torbati, S., Lisar, S.Y.S., & Zarei, M. (2012). Phytoremediation potential of duckweed (Lemna minor L.) in degradation of C.I. Acid Blue 92: Artificial neural network modeling. Ecotoxicology and Environmental Safety, 80, 291-298. doi.org/10.1016/j.ecoenv.2012.03.021.

Nasrullah, A., Khan, H., Khan, A.S., Man, Z., Muhammad, N., Khan, M.I., Naser M, N., & Abd El-Salam El-Salam. (2015). Potential biosorbent derived from Calligonum polygonoides for removal of methylene blue dye from aqueous solution. The Scientific World Journal, 1-11. doi.org/10.1155/2015/562693

Parshetti, G.K., Kalme, S.D., Saratale, G.D., & Govindwar, S.P. (2006). Biodegradation of malachite green by Kocuria rosea MTCC 1532. Acta Chimica Slovenica, 53, 492–498.

Pathania, D., Sharma, S., & Pardeep Singh, P. (2017). Removal of methylene blue by adsorption onto activated carbon developed from Ficus carica bast. King Saud University Arabian Journal of Chemistry, 10 s1445-s1451. doi.org/10.1016/j.arabjc.2013.04.021.

Phromyot, S., Suwan, N., & Meenakarn, W. (1988). Aquatic plants for aquarium decoration. Thai fisheries gazette, 41(5), 455-458.

Seif, C., Portillo, F.J.M. Osmonov, D.K. Böhler, G., Christof van der Horst, Leissner, J., Hohenfellner, R., Juenemann, K.P., & Braun, P.M. (2004). Methylene blue staining for nerve-sparing operative procedures: An animal model. Urology Research and Practice, 63, 1205–1208.

Silverstein, R.M., Webster, F.X., Kiemle, D.J. & Bryce, D.L. 2014. Spectrometric Identification of Organic Compounds (8th ed.). Wiley.

Socrates, G. 2001. Infrared and Raman Characteristic Group Frequencies: Tables and Charts (3rd ed.) J. Wiley and Sons.

Sriphen, S. (1987). Aquatic plants. Department of Botany, Faculty of Science, Kasetsart University.

Tangahu, B.V., Abdullah, S.R.S., Basri, H., Idris,M., Anuar, N., & Mukhlisin, M. (2011). A review on heavy metals (As, Pb, and Hg) uptake by plants through phytoremediation. International Journal of Chemical Engineering. ID939161, 31 p.

Thilakar, R.J., J.J. Rathi & P.M. Pillai. (2012). Phytoaccumulation of chromium and copper by Pistia stratiotes L. and Salvinia natans (L.) all. The Journal of Natural Product and Plant Resources, 2, 725–730.

Tiyapairat. Y. (2005). Building Effluent Standards. Notification of the Ministry of Natural Resources and Environment on effluent standards from buildings of certain types and size, 122, 125.

Vieira, A.P., Santana, S.A.A.., Bezerra, C.W.B., Silva, H.A.S., Chaves, J.A.P., de Melo, J.C.P., da Silva Filho, E.C., & Airoldi, C. (2009). Kinetics and thermodynamics of textile dye adsorption from aqueous solutions using babassu coconut mesocarp. Journal of hazardous materials, 166, 1272-1278. doi.org/10.1016/j.jhazmat.2008.12.043.