Antagonistic screening of Trichoderma spp. against Ganoderma boninense, a causal pathogen of basal stem rot disease in oil palm
Keywords:
Trichoderma spp., Ganoderma orbiforme, Primary screening, BiocontrolAbstract
Ganoderma orbiforme is a fungus that causes basal stem rot disease in oil palms (Elaeis guineensis). It is one of the severe problems affecting oil palm production, an economically important oil crop. The antagonistic fungus Trichoderma is an environmentally friendly biological control microorganism for controlling plant diseases. In this study, eight isolates of Trichoderma spp. were screened for inhibiting the mycelial growth of G. orbiforme K4/2 by the dual culture method. It was found that four isolates of Trichoderma spp. had a percentage of inhibition more significant than 70%, including Trichoderma sp. Z1-02 had the highest percentage of inhibition (73.26%), followed by Trichoderma sp. T1-01 (72.22%), Trichoderma sp. SP1-01 (71.87%) and Trichoderma sp. Z1-01 (71.87%). Examining antifungal metabolites in culture filtrates of Trichoderma spp. isolates SP1-01 and Z1-02 could produce β-1,3- glucanase, one of the cell wall-degrading enzymes. The β-1,3- glucanase activities of isolates SP1-01 and Z1-02 were 0.0175 U/ml and 0.0071 U/ml, respectively.
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