Isolation and Identification of Actinomycetes Producing Plant Growth-Promoting Substances from the Rhizosphere of the Cymbopogon citratus
Keywords:
Actinomycetes, Indole-3-acetic acid, Ammonia, ERIC-PCR, RAPDAbstract
Actinomycetes are a group of bacteria crucial for plants as they enhance growth, protect against diseases, and contribute to nutrient cycling in soil. This study aimed to isolate and identify actinomycetes from the rhizosphere of lemongrass and to evaluate their ability to produce indole-3-acetic acid (IAA) and ammonia (NH3). Five actinomycete strains were successfully obtained, all capable of producing IAA and NH3, with concentrations ranging from 11.71±0.96 to 43.41±0.80 µg/ml and 7.02±1.16 to 33.77±1.00 mg/ml, respectively. Strain A16 produced significantly higher amounts of IAA and NH3. The DNA fingerprints of these actinomycetes were assessed using Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and Random amplified polymorphic DNA (RAPD). Both methods generated distinct DNA fingerprints specific to individual strains. Analysis of 16S rDNA sequencing identified strain A16 as a member of the genus Streptomyces. These actinomycete strains warrant further investigation for their potential to suppress plant pathogens and promote plant growth under field conditions.
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