Effect of Chlorine Dioxide (ClO2) on sterilization in micropropagation of Leb Nok Pattani Rice

Abstract

In this research, media sterilization by autoclaving and adding Chlorine Dioxide (ClO₂) at different concentrations were compared to study the effect of sterilization procedures on the micropropagation of Leb Nok Pattani Rice. Seeds were cultured on Murashige and Skoog (MS) medium for 4 weeks. Then seedlings were cultured on MS medium supplemented with the combination of 0 and 2 mg/l BA with 0 5 10 15 20 25 ClO₂ for 1 week. It was found that MS medium supplemented with a combination of 2 mg/l BA and 25 mg/l ClO₂ gave the highest survival rate 73.3% and the highest average number of shoots 2.8 shoots/explant whereas the highest shoot length 12.3 cm. was observed on MS medium supplemented the combination 2 mg/l BA with 20 mg/l ClO₂. The highest average root number 9.9 roots/explant and the highest average root length 5.4 cm. were obtained on MS medium supplemented with 2 mg/l BA.